Detailed information of TA system TA177109

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 20223..20782
• Replicon: plasmid unnamednovel_2
• Accession: NZ_CP062880
• Organism: Escherichia coli O157:H16 strain Res13-Lact-PEA12-26

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: IMP69_RS25005
• Protein ID: WP_000421255.1
• Coordinates: 20507..20782 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: IMP69_RS25000
• Protein ID: WP_001487904.1
• Coordinates: 20223..20507 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP69_RS24990	16368..19334	-	2967	WP_000008826.1	Tn3 family transposase	-
IMP69_RS24995	19315..19938	-	624	WP_000904945.1	recombinase family protein	-
IMP69_RS25000	20223..20507	+	285	WP_001487904.1	ribbon-helix-helix domain-containing protein	Antitoxin
IMP69_RS25005	20507..20782	+	276	WP_000421255.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IMP69_RS25010	20852..21244	+	393	Protein_16	hypothetical protein	-
IMP69_RS25015	21865..25689	+	3825	WP_071528426.1	autotransporter outer membrane beta-barrel domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..77359	77359
	-	flank	IS/Tn	-	-	19315..19938	623

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10718.42 Da
• Isoelectric Point: 9.9324

>T177109 WP_000421255.1 NZ_CP062880:20507-20782 [Escherichia coli O157:H16]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRIGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T177109 NZ_CP083134:c1119129-1118392 [Bacillus thuringiensis]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTTTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACGCCTAATATTTCAGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCAATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGAGAAATATACACCACAATTATTCAAAATGTTAATCCAGCAGTTTTCGGTACGATGG
AATGGCATACGGAAGAAGAATATACAAAGAGCTTAAATGTATTTTTAGATTCATATGGGGAAAAAATTGGCGCGAAAATT
GTTGTTTTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCAGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGATG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10554.04 Da
• Isoelectric Point: 6.2191

>AT177109 WP_001487904.1 NZ_CP062880:20223-20507 [Escherichia coli O157:H16]
MKMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNHLTLEALDDVTSGQVIDHQAVQRW
ADSLGTDEPLPLPR

Nucleotide

• Length: 285 bp

>AT177109 NZ_CP083134:c1118279-1118151 [Bacillus thuringiensis]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGATAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATGC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA