Detailed information of TA system TA177101

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 60918..61543
• Replicon: plasmid unnamednovel_1
• Accession: NZ_CP062879
• Organism: Escherichia coli O157:H16 strain Res13-Lact-PEA12-26

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IMP69_RS24550
• Protein ID: WP_096973166.1
• Coordinates: 60918..61316 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q31SG2
• Locus tag: IMP69_RS24555
• Protein ID: WP_000450530.1
• Coordinates: 61316..61543 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP69_RS24515	55963..56934	+	972	WP_000817026.1	ParB/RepB/Spo0J family plasmid partition protein	-
IMP69_RS24520	57447..57698	+	252	WP_001132891.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
IMP69_RS24525	57695..57982	+	288	WP_000222773.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IMP69_RS24530	58118..58423	+	306	Protein_56	helix-turn-helix domain-containing protein	-
IMP69_RS24535	58701..59749	-	1049	Protein_57	transposase	-
IMP69_RS24540	59765..60438	-	674	Protein_58	transposase	-
IMP69_RS24545	60511..60909	+	399	Protein_59	conjugal transfer protein TraD	-
IMP69_RS24550	60918..61316	-	399	WP_096973166.1	tRNA(fMet)-specific endonuclease VapC	Toxin
IMP69_RS24555	61316..61543	-	228	WP_000450530.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pic	1..131388	131388

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14899.22 Da
• Isoelectric Point: 8.5264

>T177101 WP_096973166.1 NZ_CP062879:c61316-60918 [Escherichia coli O157:H16]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVVGLRIEDWS

Nucleotide

• Length: 399 bp

>T177101 NZ_CP083089:54735-55319 [Bacillus thuringiensis]
TTGGCTAATAAAGATAATCCTAAATTCTACACCATTTCAACTGAATATATAGATTATTTAAGAGAAACGGATAGTAAAGT
ACCTTTTAATAAAGATGAACAGCATAGTAGACCGTATGTAGGTGTATTGGAAAAAATCAATGGTCATGATTATTTTGTAC
CTTTAACATCACGTAACGATAAAAATTTTAACAGTCAGGTCAGTGTGAAATTATTCGATACTGATGAAAAGCGAATTGGA
GTTTTATTGGTTAATAACATGATTCCAGTCCCAGAAAAAGAGTGTAAAGAAATTGATATAGCAGAAAAAACAGCAGCAGA
CCCTCAGTATGGTAATTTGATGTTAAAACAGTATTTATTTTTAAAAGAAAATATGGATCGAGTTACTAATAAAGTGGAAA
AAGTATATAAAGATGTAACTGTACAAGGAAAGCCTTCACATAAACAAAAATTCTTAAAAGGAGTTTGTTGTGATTTCCCT
AAATTAGAAGAAAAGTGCCAAGAATATAAAGAAAGAGATCAAGCTAAAGAAAGAGATAAAGCTAGAAGAATAGCATATAT
GCGTCAAATGGGGCGTGAGAGATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8535.59 Da
• Isoelectric Point: 4.7513

>AT177101 WP_000450530.1 NZ_CP062879:c61543-61316 [Escherichia coli O157:H16]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT177101 NZ_CP083089:54577-54673 [Bacillus thuringiensis]
GTAACCTTACTTTAGTAGGTGCGAAAAAATTGGTGTAACTCTACCGTAGTGAGTACCAAAAAATTGGTGTAGCCTTACCG
TAGTAGGCGCTAAAAAA

Structures

Antitoxin

• AlphaFold DB: Q31SG2