Detailed information of TA system TA177100

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 57447..57982
• Replicon: plasmid unnamednovel_1
• Accession: NZ_CP062879
• Organism: Escherichia coli O157:H16 strain Res13-Lact-PEA12-26

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: IMP69_RS24525
• Protein ID: WP_000222773.1
• Coordinates: 57695..57982 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A8H9KB11
• Locus tag: IMP69_RS24520
• Protein ID: WP_001132891.1
• Coordinates: 57447..57698 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP69_RS24495	52697..52993	-	297	WP_000412343.1	helix-turn-helix domain-containing protein	-
IMP69_RS24500	53042..53194	-	153	WP_001231511.1	hypothetical protein	-
IMP69_RS24505	53279..54447	+	1169	WP_114311086.1	IS3 family transposase	-
IMP69_RS24510	54797..55963	+	1167	WP_000523852.1	plasmid-partitioning protein SopA	-
IMP69_RS24515	55963..56934	+	972	WP_000817026.1	ParB/RepB/Spo0J family plasmid partition protein	-
IMP69_RS24520	57447..57698	+	252	WP_001132891.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
IMP69_RS24525	57695..57982	+	288	WP_000222773.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IMP69_RS24530	58118..58423	+	306	Protein_56	helix-turn-helix domain-containing protein	-
IMP69_RS24535	58701..59749	-	1049	Protein_57	transposase	-
IMP69_RS24540	59765..60438	-	674	Protein_58	transposase	-
IMP69_RS24545	60511..60909	+	399	Protein_59	conjugal transfer protein TraD	-
IMP69_RS24550	60918..61316	-	399	WP_096973166.1	tRNA(fMet)-specific endonuclease VapC	-
IMP69_RS24555	61316..61543	-	228	WP_000450530.1	toxin-antitoxin system antitoxin VapB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pic	1..131388	131388
	-	inside	IScluster/Tn	-	-	47078..53581	6503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11221.29 Da
• Isoelectric Point: 10.5832

>T177100 WP_000222773.1 NZ_CP062879:57695-57982 [Escherichia coli O157:H16]
MTYTVKFRDDALKEWMKLDKSIQQQFAKKLKKCSENPHIPSAKLRELKNCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERNNVYNLASERMR

Nucleotide

• Length: 288 bp

>T177100 NZ_CP083085:c3584993-3584256 [Bacillus thuringiensis]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
AGAGTTGTACGAGGAGAAGAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACGCCTAATATTTCAGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCCATAAAACGATC
ATTAATTGCATCTAAAGCAAGAGGAGAAATATACACGACAATTATTCAAAATGTTAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGCGCGAAAATT
GTTGTTTTTGAAGCTGCGAAGGAATTAAATACAAACTTTCGTGGTATTCGCTCACAATTTAGTATTATCGTCCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9181.39 Da
• Isoelectric Point: 4.0780

>AT177100 WP_001132891.1 NZ_CP062879:57447-57698 [Escherichia coli O157:H16]
MPNIILSDTSASVSELKKNPMATVGAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT177100 NZ_CP083085:c3584143-3584015 [Bacillus thuringiensis]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGATAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA