Detailed information of TA system TA177098

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 1777..2534
• Replicon: plasmid unnamednovel_1
• Accession: NZ_CP062879
• Organism: Escherichia coli O157:H16 strain Res13-Lact-PEA12-26

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A0L6ZF90
• Locus tag: IMP69_RS24265
• Protein ID: WP_000501972.1
• Coordinates: 2049..2534 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: IMP69_RS24260
• Protein ID: WP_072141942.1
• Coordinates: 1777..2061 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP69_RS24255	1..858	+	858	WP_195686735.1	incFII family plasmid replication initiator RepA	-
IMP69_RS24260	1777..2061	+	285	WP_072141942.1	DUF1778 domain-containing protein	Antitoxin
IMP69_RS24265	2049..2534	+	486	WP_000501972.1	GNAT family N-acetyltransferase	Toxin
IMP69_RS24270	3154..4256	-	1103	Protein_4	transposase	-
IMP69_RS24275	4256..4621	-	366	WP_195686736.1	hypothetical protein	-
IMP69_RS24280	4893..5051	-	159	WP_195686737.1	hypothetical protein	-
IMP69_RS24285	5116..5813	-	698	Protein_7	IS1 family transposase	-
IMP69_RS24290	5847..6185	-	339	WP_195686738.1	hypothetical protein	-
IMP69_RS24295	6347..7384	+	1038	Protein_9	IS21 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pic	1..131388	131388

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (31-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-93)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17630.39 Da
• Isoelectric Point: 9.9107

>T177098 WP_000501972.1 NZ_CP062879:2049-2534 [Escherichia coli O157:H16]
MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQALGATRTFVVCRKGTQQVVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDASFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTESAKQFYIHHGFTPSKTQLQTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T177098 NZ_CP083078:c31169-30753 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10973.61 Da
• Isoelectric Point: 4.5625

>AT177098 WP_072141942.1 NZ_CP062879:1777-2061 [Escherichia coli O157:H16]
MIYGGFMKSDVQLNLRARESQRILIDTAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADEP
AIEKLLARKPQWDV

Nucleotide

• Length: 285 bp

>AT177098 NZ_CP083078:c31396-31166 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0L6ZF90