Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3908778..3909432 Replicon chromosome
Accession NZ_CP062876
Organism Escherichia coli O157:H16 strain Res13-Lact-PEA12-26

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag IMP69_RS19130 Protein ID WP_000244777.1
Coordinates 3909025..3909432 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag IMP69_RS19125 Protein ID WP_000354046.1
Coordinates 3908778..3909044 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP69_RS19100 3903947..3904690 + 744 WP_000951964.1 SDR family oxidoreductase -
IMP69_RS19105 3904747..3906180 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
IMP69_RS19110 3906225..3906536 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
IMP69_RS19115 3906700..3907359 + 660 WP_000250274.1 hemolysin III family protein -
IMP69_RS19120 3907555..3908535 - 981 WP_000886071.1 tRNA-modifying protein YgfZ -
IMP69_RS19125 3908778..3909044 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
IMP69_RS19130 3909025..3909432 + 408 WP_000244777.1 toxin CptA Toxin
IMP69_RS19135 3909472..3909993 - 522 WP_001055874.1 flavodoxin FldB -
IMP69_RS19140 3910105..3911001 + 897 WP_000806630.1 site-specific tyrosine recombinase XerD -
IMP69_RS19145 3911026..3911736 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IMP69_RS19150 3911742..3913475 + 1734 WP_000813185.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T177090 WP_000244777.1 NZ_CP062876:3909025-3909432 [Escherichia coli O157:H16]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T177090 NZ_CP083075:2066514-2066616 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT177090 WP_000354046.1 NZ_CP062876:3908778-3909044 [Escherichia coli O157:H16]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT177090 NZ_CP083075:c2066623-2066478 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References