Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3292403..3293034 Replicon chromosome
Accession NZ_CP062876
Organism Escherichia coli O157:H16 strain Res13-Lact-PEA12-26

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag IMP69_RS16025 Protein ID WP_001259385.1
Coordinates 3292403..3292678 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag IMP69_RS16030 Protein ID WP_000593555.1
Coordinates 3292675..3293034 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP69_RS16010 3287407..3288474 + 1068 WP_000361491.1 HlyD family efflux transporter periplasmic adaptor subunit -
IMP69_RS16015 3288471..3291206 + 2736 WP_000149151.1 ribosome-associated ATPase/putative transporter RbbA -
IMP69_RS16020 3291206..3292330 + 1125 WP_001488398.1 ABC transporter permease -
IMP69_RS16025 3292403..3292678 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
IMP69_RS16030 3292675..3293034 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
IMP69_RS16035 3293154..3293555 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
IMP69_RS16040 3293561..3294367 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
IMP69_RS16045 3294364..3295128 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
IMP69_RS16050 3295128..3295961 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
IMP69_RS16055 3295958..3296902 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3284525..3293034 8509


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T177087 WP_001259385.1 NZ_CP062876:3292403-3292678 [Escherichia coli O157:H16]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T177087 NZ_CP083075:949751-950161 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT177087 WP_000593555.1 NZ_CP062876:3292675-3293034 [Escherichia coli O157:H16]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT177087 NZ_CP083075:949504-949770 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References