Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2390664..2391240 Replicon chromosome
Accession NZ_CP062876
Organism Escherichia coli O157:H16 strain Res13-Lact-PEA12-26

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag IMP69_RS11865 Protein ID WP_001297643.1
Coordinates 2390953..2391240 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag IMP69_RS11860 Protein ID WP_000063156.1
Coordinates 2390664..2390966 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP69_RS11845 2387260..2389410 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
IMP69_RS11850 2389460..2389663 + 204 WP_000467859.1 YbdD/YjiX family protein -
IMP69_RS11855 2389674..2390630 + 957 WP_001297640.1 GTPase -
IMP69_RS11860 2390664..2390966 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
IMP69_RS11865 2390953..2391240 - 288 WP_001297643.1 BrnT family toxin Toxin
IMP69_RS11870 2391439..2392136 - 698 Protein_2320 IS1 family transposase -
IMP69_RS11875 2392193..2392288 + 96 Protein_2321 dienelactone hydrolase family protein -
IMP69_RS11880 2392434..2393666 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
IMP69_RS11885 2393707..2394987 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T177085 WP_001297643.1 NZ_CP062876:c2391240-2390953 [Escherichia coli O157:H16]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T177085 NZ_CP083075:334338-334706 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGACAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT177085 WP_000063156.1 NZ_CP062876:c2390966-2390664 [Escherichia coli O157:H16]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT177085 NZ_CP083075:334120-334341 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References