Detailed information of TA system TA177082

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 1100553..1101034
• Replicon: chromosome
• Accession: NZ_CP062876
• Organism: Escherichia coli O157:H16 strain Res13-Lact-PEA12-26

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7YS45
• Locus tag: IMP69_RS05620
• Protein ID: WP_014966210.1
• Coordinates: 1100744..1101034 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: A0A0P7NPJ2
• Locus tag: IMP69_RS05615
• Protein ID: WP_000100895.1
• Coordinates: 1100553..1100744 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP69_RS05580	1096498..1096914	-	417	WP_000124819.1	DUF977 family protein	-
IMP69_RS05585	1096930..1097643	-	714	WP_000450632.1	DUF1627 domain-containing protein	-
IMP69_RS05590	1097677..1098219	-	543	WP_073972894.1	replication protein	-
IMP69_RS05595	1098131..1099171	-	1041	WP_001262421.1	hypothetical protein	-
IMP69_RS05600	1099243..1099668	-	426	WP_000693898.1	hypothetical protein	-
IMP69_RS05605	1099652..1099927	-	276	WP_001048457.1	helix-turn-helix domain-containing protein	-
IMP69_RS05610	1100035..1100535	+	501	WP_000824162.1	helix-turn-helix transcriptional regulator	-
IMP69_RS05615	1100553..1100744	+	192	WP_000100895.1	hypothetical protein	Antitoxin
IMP69_RS05620	1100744..1101034	+	291	WP_014966210.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IMP69_RS05625	1101304..1101459	+	156	WP_000379589.1	DUF1391 family protein	-
IMP69_RS05630	1101619..1101837	+	219	WP_001171969.1	hypothetical protein	-
IMP69_RS05635	1101841..1102005	-	165	WP_001331716.1	hypothetical protein	-
IMP69_RS05640	1102406..1102594	+	189	WP_000449192.1	cell division inhibition protein DicB	-
IMP69_RS05645	1102591..1102782	+	192	WP_001090203.1	DUF1482 family protein	-
IMP69_RS05650	1102875..1105346	+	2472	WP_000048451.1	exonuclease	-
IMP69_RS05655	1105408..1105677	+	270	WP_000003742.1	excisionase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1058465..1116639	58174

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11176.05 Da
• Isoelectric Point: 9.9854

>T177082 WP_014966210.1 NZ_CP062876:1100744-1101034 [Escherichia coli O157:H16]
MLPILWLPSARDDLRQIVAYIAKENPPAARRLKIRIETSVLSLTEHPYLYPPSERVPSLREIVTHPNYIILYRVTASNIE
IVNVVHSRRQYPNKTC

Nucleotide

• Length: 291 bp

>T177082 NZ_CP083070:c5236877-5236494 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7003.80 Da
• Isoelectric Point: 4.0270

>AT177082 WP_000100895.1 NZ_CP062876:1100553-1100744 [Escherichia coli O157:H16]
MSTALSPIISEFETVEQENSYNEWLRAKVAASLSDPRPAIPHDEVMAEMENLIAQLAATNRSE

Nucleotide

• Length: 192 bp

>AT177082 NZ_CP083070:c5237119-5236877 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A0D7YS45

Antitoxin

• AlphaFold DB: A0A0P7NPJ2