Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 15725..16260 Replicon plasmid unnamed539
Accession NZ_CP062874
Organism Escherichia coli strain Res13-Lact-PEA14-20

Toxin (Protein)


Gene name relE Uniprot ID Q3YTD6
Locus tag IMP76_RS25080 Protein ID WP_000222766.1
Coordinates 15725..16012 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0VIP2
Locus tag IMP76_RS25085 Protein ID WP_001132900.1
Coordinates 16009..16260 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP76_RS25060 13310..13501 + 192 Protein_9 IS91 family transposase -
IMP76_RS25065 13469..13684 - 216 Protein_10 helix-turn-helix transcriptional regulator -
IMP76_RS25070 13780..14037 + 258 Protein_11 IS3 family transposase -
IMP76_RS25075 14244..15456 + 1213 Protein_12 IS3 family transposase -
IMP76_RS25080 15725..16012 - 288 WP_000222766.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IMP76_RS25085 16009..16260 - 252 WP_001132900.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
IMP76_RS25090 17214..17321 - 108 Protein_15 incFII family plasmid replication initiator RepA -
IMP76_RS25095 17344..17592 - 249 WP_000083841.1 replication regulatory protein RepA -
IMP76_RS25100 18082..18837 - 756 WP_000065240.1 IS21-like element ISEc10 family helper ATPase IstB -
IMP76_RS25105 18834..20333 - 1500 WP_000174402.1 IS21-like element ISEc10 family transposase -
IMP76_RS25110 20829..21044 - 216 Protein_19 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD vat / exeG / exeE / stcE 1..123046 123046
- inside IScluster/Tn - vat 7766..20333 12567


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11147.23 Da        Isoelectric Point: 10.5832

>T177067 WP_000222766.1 NZ_CP062874:c16012-15725 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Download         Length: 288 bp

>T177067 NZ_CP083067:11183-11599 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9211.41 Da        Isoelectric Point: 4.0780

>AT177067 WP_001132900.1 NZ_CP062874:c16260-16009 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Download         Length: 252 bp

>AT177067 NZ_CP083067:10956-11186 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CDZ2

Antitoxin

Source ID Structure
AlphaFold DB V0VIP2

References