Detailed information of TA system TA177052

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1829927..1830510
• Replicon: chromosome
• Accession: NZ_CP062871
• Organism: Escherichia coli strain Res13-Lact-PEA14-20

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: U9XFN8
• Locus tag: IMP76_RS09070
• Protein ID: WP_000254745.1
• Coordinates: 1829927..1830262 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: IMP76_RS09075
• Protein ID: WP_096942168.1
• Coordinates: 1830262..1830510 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP76_RS09055	1825814..1827112	-	1299	WP_000036723.1	phosphopyruvate hydratase	-
IMP76_RS09060	1827200..1828837	-	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
IMP76_RS09065	1829065..1829856	-	792	WP_001071639.1	nucleoside triphosphate pyrophosphohydrolase	-
IMP76_RS09070	1829927..1830262	-	336	WP_000254745.1	endoribonuclease MazF	Toxin
IMP76_RS09075	1830262..1830510	-	249	WP_096942168.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IMP76_RS09080	1830588..1832822	-	2235	WP_000226815.1	GTP pyrophosphokinase	-
IMP76_RS09085	1832870..1834171	-	1302	WP_000046800.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12158.08 Da
• Isoelectric Point: 8.4777

>T177052 WP_000254745.1 NZ_CP062871:c1830262-1829927 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T177052 NZ_CP083063:c4671421-4670888 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9387.80 Da
• Isoelectric Point: 4.4665

>AT177052 WP_096942168.1 NZ_CP062871:c1830510-1830262 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPMRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT177052 NZ_CP083063:c4671698-4671432 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0XYM3