Detailed information of TA system TA177051

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1534052..1534677
• Replicon: chromosome
• Accession: NZ_CP062871
• Organism: Escherichia coli strain Res13-Lact-PEA14-20

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IMP76_RS07610
• Protein ID: WP_000911330.1
• Coordinates: 1534052..1534450 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: IMP76_RS07615
• Protein ID: WP_000450524.1
• Coordinates: 1534450..1534677 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP76_RS07590	1529930..1530130	+	201	WP_000383836.1	YpfN family protein	-
IMP76_RS07595	1530240..1530938	-	699	WP_000679812.1	esterase	-
IMP76_RS07600	1531012..1533027	-	2016	WP_096942028.1	tRNA cytosine(34) acetyltransferase TmcA	-
IMP76_RS07605	1533042..1533905	-	864	WP_001267507.1	neutral zinc metallopeptidase	-
IMP76_RS07610	1534052..1534450	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
IMP76_RS07615	1534450..1534677	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
IMP76_RS07620	1534831..1535544	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
IMP76_RS07625	1535757..1536791	-	1035	WP_001358397.1	outer membrane protein assembly factor BamC	-
IMP76_RS07630	1536808..1537686	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
IMP76_RS07635	1537832..1538404	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
IMP76_RS07640	1538404..1538874	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T177051 WP_000911330.1 NZ_CP062871:c1534450-1534052 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T177051 NZ_CP083063:4037976-4038194 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT177051 WP_000450524.1 NZ_CP062871:c1534677-1534450 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT177051 NZ_CP083063:4037575-4037949 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A829CM33