Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 374499..375137 Replicon chromosome
Accession NZ_CP062871
Organism Escherichia coli strain Res13-Lact-PEA14-20

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag IMP76_RS01905 Protein ID WP_000813794.1
Coordinates 374499..374675 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag IMP76_RS01910 Protein ID WP_001270286.1
Coordinates 374721..375137 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP76_RS01885 370118..371332 - 1215 WP_001307190.1 BenE family transporter YdcO -
IMP76_RS01890 371385..371921 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
IMP76_RS01895 371994..373955 + 1962 WP_096942282.1 23S rRNA 5-hydroxycytidine C2501 synthase -
IMP76_RS01900 374047..374277 - 231 WP_000494244.1 YncJ family protein -
IMP76_RS01905 374499..374675 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
IMP76_RS01910 374721..375137 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
IMP76_RS01915 375216..376622 + 1407 WP_096942412.1 PLP-dependent aminotransferase family protein -
IMP76_RS01920 376867..378012 + 1146 WP_000047462.1 ABC transporter substrate-binding protein -
IMP76_RS01925 378030..379043 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
IMP76_RS01930 379044..379985 + 942 WP_096942413.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 368930..370078 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T177040 WP_000813794.1 NZ_CP062871:374499-374675 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T177040 NZ_CP083055:4074295-4074513 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT177040 WP_001270286.1 NZ_CP062871:374721-375137 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT177040 NZ_CP083055:4073894-4074268 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References