Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 130462..130987 Replicon plasmid unnamed973
Accession NZ_CP062870
Organism Escherichia coli strain Res13-Lact-PEB01-20

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag IMP84_RS23660 Protein ID WP_001159868.1
Coordinates 130462..130767 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag IMP84_RS23665 Protein ID WP_000813634.1
Coordinates 130769..130987 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP84_RS23645 126394..127560 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
IMP84_RS23650 128148..128903 - 756 WP_000852146.1 replication initiation protein RepE -
IMP84_RS23655 129655..130461 - 807 WP_195707259.1 site-specific integrase -
IMP84_RS23660 130462..130767 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
IMP84_RS23665 130769..130987 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
IMP84_RS23670 131555..132067 + 513 WP_000151784.1 hypothetical protein -
IMP84_RS23675 132101..133234 - 1134 WP_000545984.1 DUF3800 domain-containing protein -
IMP84_RS23680 133401..134174 - 774 WP_000905949.1 hypothetical protein -
IMP84_RS23685 134187..134687 - 501 WP_000528932.1 hypothetical protein -
IMP84_RS23690 134952..135182 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
IMP84_RS23695 135179..135595 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / sitABCD iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA 1..162467 162467


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T177037 WP_001159868.1 NZ_CP062870:c130767-130462 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T177037 NZ_CP083055:1970793-1970895 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT177037 WP_000813634.1 NZ_CP062870:c130987-130769 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT177037 NZ_CP083055:c1970902-1970757 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References