Detailed information of TA system TA177032

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 83654..84279
• Replicon: plasmid unnamed973
• Accession: NZ_CP062870
• Organism: Escherichia coli strain Res13-Lact-PEB01-20

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IMP84_RS23360
• Protein ID: WP_000911313.1
• Coordinates: 83881..84279 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: IMP84_RS23355
• Protein ID: WP_000450520.1
• Coordinates: 83654..83881 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP84_RS23355	83654..83881	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
IMP84_RS23360	83881..84279	+	399	WP_000911313.1	tRNA(fMet)-specific endonuclease VapC	Toxin
IMP84_RS23365	84288..86441	-	2154	WP_000009324.1	type IV conjugative transfer system coupling protein TraD	-
IMP84_RS23370	86694..87425	-	732	WP_023565183.1	complement resistance protein TraT	-
IMP84_RS23375	87457..87954	-	498	WP_000605857.1	entry exclusion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..162467	162467

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T177032 WP_000911313.1 NZ_CP062870:83881-84279 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T177032 NZ_CP083051:c4902108-4901191 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCAATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTGAGCCATAATCGGCCTCTGGTTGAAGTGCTGAGTCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT177032 WP_000450520.1 NZ_CP062870:83654-83881 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT177032 NZ_CP083051:c4902872-4902114 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCTTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: V0VCB2