Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 131099..131624 Replicon plasmid unnamed972
Accession NZ_CP062867
Organism Escherichia coli O39:H21 strain Res13-Lact-PEB08-01

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag IMP90_RS24195 Protein ID WP_001159871.1
Coordinates 131099..131404 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag IMP90_RS24200 Protein ID WP_000813630.1
Coordinates 131406..131624 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP90_RS24180 127061..128227 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
IMP90_RS24185 128815..129570 - 756 WP_000852146.1 replication initiation protein RepE -
IMP90_RS24190 130292..131098 - 807 WP_000016970.1 site-specific integrase -
IMP90_RS24195 131099..131404 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
IMP90_RS24200 131406..131624 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
IMP90_RS24205 132252..132482 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
IMP90_RS24210 132479..132895 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
IMP90_RS24215 132970..134535 + 1566 WP_001128474.1 AAA family ATPase -
IMP90_RS24220 134520..135542 + 1023 WP_000361402.1 helicase UvrD -
IMP90_RS24225 135796..136482 - 687 Protein_142 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iroB / iroC / iroD / iroE / iroN / vat / iucA / iucB / iucC / iucD / iutA 1..155756 155756
- flank IS/Tn sitABCD - 135796..140287 4491


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T176995 WP_001159871.1 NZ_CP062867:c131404-131099 [Escherichia coli O39:H21]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T176995 NZ_CP083043:2055080-2055182 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT176995 WP_000813630.1 NZ_CP062867:c131624-131406 [Escherichia coli O39:H21]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT176995 NZ_CP083043:c2055189-2055044 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References