Detailed information of TA system TA176984

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 27015..27562
• Replicon: plasmid unnamed972
• Accession: NZ_CP062867
• Organism: Escherichia coli O39:H21 strain Res13-Lact-PEB08-01

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: IMP90_RS23615
• Protein ID: WP_000969990.1
• Coordinates: 27015..27296 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: IMP90_RS23620
• Protein ID: WP_000079941.1
• Coordinates: 27293..27562 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP90_RS23595	23280..24314	-	1035	WP_001190234.1	3-deoxy-7-phosphoheptulonate synthase	-
IMP90_RS23600	24873..25220	-	348	WP_000142437.1	hypothetical protein	-
IMP90_RS23605	25522..26004	-	483	WP_001311056.1	hypothetical protein	-
IMP90_RS23610	26121..26969	-	849	WP_001058005.1	3'-5' exonuclease	-
IMP90_RS23615	27015..27296	-	282	WP_000969990.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IMP90_RS23620	27293..27562	-	270	WP_000079941.1	hypothetical protein	Antitoxin
IMP90_RS23625	28084..29320	+	1237	Protein_22	IS3 family transposase	-
IMP90_RS23630	29517..30791	+	1275	WP_172836775.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iucA / iucB / iucC / iucD / iutA	1..155756	155756

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10930.61 Da
• Isoelectric Point: 8.4615

>T176984 WP_000969990.1 NZ_CP062867:c27296-27015 [Escherichia coli O39:H21]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGYSASNLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTVQKWP

Nucleotide

• Length: 282 bp

>T176984 NZ_CP083036:1777606-1777938 [Klebsiella pneumoniae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGACTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGGACTATC
GATATGGCGGCCCGGAAAGGCAAGCGTCTGGAACGCATACCTGACGTCGTTGTCAACGAGGTGCTTGCCAGGCTGGATGC
GATGCTGAGTTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT176984 WP_000079941.1 NZ_CP062867:c27562-27293 [Escherichia coli O39:H21]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT176984 NZ_CP083036:1777348-1777605 [Klebsiella pneumoniae]
ATGTATACAACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A629TXC5