Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4258852..4259483 Replicon chromosome
Accession NZ_CP062865
Organism Escherichia coli O39:H21 strain Res13-Lact-PEB08-01

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag IMP90_RS20595 Protein ID WP_001259388.1
Coordinates 4259208..4259483 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag IMP90_RS20590 Protein ID WP_000593555.1
Coordinates 4258852..4259211 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP90_RS20565 4254984..4255928 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
IMP90_RS20570 4255925..4256758 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
IMP90_RS20575 4256758..4257522 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
IMP90_RS20580 4257519..4258325 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
IMP90_RS20585 4258331..4258732 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
IMP90_RS20590 4258852..4259211 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
IMP90_RS20595 4259208..4259483 - 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
IMP90_RS20600 4259556..4260680 - 1125 WP_001314210.1 ABC transporter permease -
IMP90_RS20605 4260680..4263415 - 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
IMP90_RS20610 4263412..4264479 - 1068 WP_000361485.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T176977 WP_001259388.1 NZ_CP062865:c4259483-4259208 [Escherichia coli O39:H21]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T176977 NZ_CP083032:c5140164-5139781 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGTACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGAAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT176977 WP_000593555.1 NZ_CP062865:c4259211-4258852 [Escherichia coli O39:H21]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT176977 NZ_CP083032:c5140406-5140164 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References