Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3663650..3664304 Replicon chromosome
Accession NZ_CP062865
Organism Escherichia coli O39:H21 strain Res13-Lact-PEB08-01

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag IMP90_RS17620 Protein ID WP_000244772.1
Coordinates 3663650..3664057 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag IMP90_RS17625 Protein ID WP_000354046.1
Coordinates 3664038..3664304 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP90_RS17600 3659607..3661340 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
IMP90_RS17605 3661346..3662056 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IMP90_RS17610 3662081..3662977 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
IMP90_RS17615 3663089..3663610 + 522 WP_001055874.1 flavodoxin FldB -
IMP90_RS17620 3663650..3664057 - 408 WP_000244772.1 protein YgfX Toxin
IMP90_RS17625 3664038..3664304 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
IMP90_RS17630 3664547..3665527 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
IMP90_RS17635 3665604..3666263 - 660 WP_000250274.1 hemolysin III family protein -
IMP90_RS17640 3666427..3666738 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
IMP90_RS17645 3666783..3668216 + 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
IMP90_RS17650 3668273..3669016 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T176975 WP_000244772.1 NZ_CP062865:c3664057-3663650 [Escherichia coli O39:H21]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T176975 NZ_CP083032:c4662102-4661818 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT176975 WP_000354046.1 NZ_CP062865:c3664304-3664038 [Escherichia coli O39:H21]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT176975 NZ_CP083032:c4662334-4662092 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References