Detailed information of TA system TA176973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3215355..3215980
• Replicon: chromosome
• Accession: NZ_CP062865
• Organism: Escherichia coli O39:H21 strain Res13-Lact-PEB08-01

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IMP90_RS15460
• Protein ID: WP_000911330.1
• Coordinates: 3215355..3215753 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: IMP90_RS15465
• Protein ID: WP_000450524.1
• Coordinates: 3215753..3215980 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP90_RS15440	3211262..3211462	+	201	WP_000383836.1	YpfN family protein	-
IMP90_RS15445	3211543..3212241	-	699	WP_000679812.1	esterase	-
IMP90_RS15450	3212315..3214330	-	2016	WP_000829292.1	tRNA cytosine(34) acetyltransferase TmcA	-
IMP90_RS15455	3214345..3215208	-	864	WP_001267498.1	neutral zinc metallopeptidase	-
IMP90_RS15460	3215355..3215753	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
IMP90_RS15465	3215753..3215980	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
IMP90_RS15470	3216134..3216847	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
IMP90_RS15475	3217060..3218094	-	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
IMP90_RS15480	3218111..3218989	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
IMP90_RS15485	3219135..3219707	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
IMP90_RS15490	3219707..3220177	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3206481..3215980	9499

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T176973 WP_000911330.1 NZ_CP062865:c3215753-3215355 [Escherichia coli O39:H21]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T176973 NZ_CP083032:3960262-3960480 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT176973 WP_000450524.1 NZ_CP062865:c3215980-3215753 [Escherichia coli O39:H21]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT176973 NZ_CP083032:3959861-3960235 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACTTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A829CM33