Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1065848..1066466 Replicon chromosome
Accession NZ_CP062865
Organism Escherichia coli O39:H21 strain Res13-Lact-PEB08-01

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag IMP90_RS05045 Protein ID WP_001291435.1
Coordinates 1065848..1066066 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag IMP90_RS05050 Protein ID WP_000344800.1
Coordinates 1066092..1066466 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP90_RS05015 1061022..1061642 + 621 WP_001267012.1 recombinase family protein -
IMP90_RS05020 1061860..1062087 + 228 WP_024183738.1 DUF2188 domain-containing protein -
IMP90_RS05025 1062431..1062784 + 354 WP_000878141.1 DUF1428 family protein -
IMP90_RS05030 1062826..1064376 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
IMP90_RS05035 1064540..1065010 - 471 WP_000136192.1 YlaC family protein -
IMP90_RS05040 1065126..1065677 - 552 WP_000102564.1 maltose O-acetyltransferase -
IMP90_RS05045 1065848..1066066 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
IMP90_RS05050 1066092..1066466 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
IMP90_RS05055 1066773..1068001 + 1229 WP_089541817.1 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T176955 WP_001291435.1 NZ_CP062865:c1066066-1065848 [Escherichia coli O39:H21]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T176955 NZ_CP083021:c5352079-5351696 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT176955 WP_000344800.1 NZ_CP062865:c1066466-1066092 [Escherichia coli O39:H21]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT176955 NZ_CP083021:c5352321-5352079 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References