Detailed information of TA system TA176942

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2433688..2434326
• Replicon: chromosome
• Accession: NZ_CP062863
• Organism: Escherichia coli strain Res13-Lact-PEB14-36

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: IMP96_RS12900
• Protein ID: WP_000813794.1
• Coordinates: 2434150..2434326 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: IMP96_RS12895
• Protein ID: WP_001270286.1
• Coordinates: 2433688..2434104 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP96_RS12875	2428840..2429781	-	942	WP_001251313.1	ABC transporter permease	-
IMP96_RS12880	2429782..2430795	-	1014	WP_000220399.1	ABC transporter ATP-binding protein	-
IMP96_RS12885	2430813..2431958	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
IMP96_RS12890	2432203..2433609	-	1407	WP_000760585.1	PLP-dependent aminotransferase family protein	-
IMP96_RS12895	2433688..2434104	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
IMP96_RS12900	2434150..2434326	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
IMP96_RS12905	2434486..2435183	+	698	Protein_2290	IS1 family transposase	-
IMP96_RS12910	2435239..2435682	+	444	Protein_2291	IS200/IS605 family transposase	-
IMP96_RS12915	2435700..2436368	-	669	WP_001261013.1	DUF3313 domain-containing protein	-
IMP96_RS12920	2436671..2437264	-	594	WP_000587030.1	tellurite resistance methyltransferase TehB	-
IMP96_RS12925	2437261..2438253	-	993	WP_001300478.1	dicarboxylate transporter/tellurite-resistance protein TehA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2429782..2440175	10393

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T176942 WP_000813794.1 NZ_CP062863:c2434326-2434150 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T176942 NZ_CP083016:c4736683-4736150 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTTGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGTATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT176942 WP_001270286.1 NZ_CP062863:c2434104-2433688 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT176942 NZ_CP083016:c4736960-4736694 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• PDB: 6HPB