176919

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 86636..87237 Replicon plasmid unnamed1009
Accession NZ_CP062860
Organism Escherichia coli strain Res13-Lact-PEB14-36

Toxin (Protein)


Gene name doc Uniprot ID F4TND7
Locus tag IMP96_RS00475 Protein ID WP_001216030.1
Coordinates 86857..87237 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag IMP96_RS00470 Protein ID WP_001190712.1
Coordinates 86636..86857 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP96_RS00460 83704..84900 - 1197 WP_094316228.1 restriction endonuclease subunit S -
IMP96_RS00465 84897..86453 - 1557 WP_096314329.1 type I restriction-modification system subunit M -
IMP96_RS00470 86636..86857 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
IMP96_RS00475 86857..87237 + 381 WP_001216030.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
IMP96_RS00480 87242..87421 + 180 WP_001436571.1 hypothetical protein -
IMP96_RS00485 87449..88492 + 1044 WP_000648833.1 DUF968 domain-containing protein -
IMP96_RS00490 88581..89033 + 453 WP_001326849.1 late promoter-activating protein -
IMP96_RS00495 89119..90312 + 1194 WP_064670834.1 hypothetical protein -
IMP96_RS00500 90312..91796 + 1485 WP_000124150.1 terminase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..97512 97512


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(4-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13601.29 Da        Isoelectric Point: 5.1408

>T176919 WP_001216030.1 NZ_CP062860:86857-87237 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEDITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T176919 NZ_CP083007:c4845216-4844932 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT176919 WP_001190712.1 NZ_CP062860:86636-86857 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT176919 NZ_CP083007:c4845448-4845206 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1M1W8Q3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References