Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 53237..53784 Replicon plasmid unnamed973
Accession NZ_CP062858
Organism Escherichia coli O20:H12 strain Res13-Lact-PEB17-18

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag IMP99_RS24660 Protein ID WP_000969996.1
Coordinates 53237..53518 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID E1QMW3
Locus tag IMP99_RS24665 Protein ID WP_000079941.1
Coordinates 53515..53784 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP99_RS24630 48667..48927 + 261 WP_000343091.1 hypothetical protein -
IMP99_RS24635 48924..49514 + 591 WP_001518980.1 hypothetical protein -
IMP99_RS24640 49534..49791 - 258 WP_000142431.1 hypothetical protein -
IMP99_RS24645 49920..50255 + 336 WP_001080729.1 colicin 1A immunity protein -
IMP99_RS24650 50277..52157 - 1881 WP_001283335.1 colicin -
IMP99_RS24655 52343..53191 - 849 WP_001057989.1 3'-5' exonuclease -
IMP99_RS24660 53237..53518 - 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IMP99_RS24665 53515..53784 - 270 WP_000079941.1 hypothetical protein Antitoxin
IMP99_RS24670 54428..57394 - 2967 WP_001138014.1 Tn3-like element TnAs1 family transposase -
IMP99_RS24675 57398..57958 - 561 WP_001161490.1 recombinase family protein -
IMP99_RS24680 58134..58484 - 351 WP_000454193.1 DUF3330 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA5 / blaTEM-1B / sitABCD / aph(6)-Id / aph(3'')-Ib / sul2 iroB / iroC / iroD / iroE / iroN / iucA / iucB / iucC / iucD / iutA 1..150153 150153


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T176918 WP_000969996.1 NZ_CP062858:c53518-53237 [Escherichia coli O20:H12]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T176918 NZ_CP083007:c4743372-4742839 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10252.58 Da        Isoelectric Point: 7.2057

>AT176918 WP_000079941.1 NZ_CP062858:c53784-53515 [Escherichia coli O20:H12]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT176918 NZ_CP083007:c4743649-4743383 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A629TXC5

References