Detailed information of TA system TA176917

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 41278..41654
• Replicon: plasmid unnamed973
• Accession: NZ_CP062858
• Organism: Escherichia coli O20:H12 strain Res13-Lact-PEB17-18

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: IMP99_RS24580
• Protein ID: WP_001514250.1
• Coordinates: 41278..41460 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: IMP99_RS24585
• Protein ID: WP_001324224.1
• Coordinates: 41457..41654 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP99_RS24570	39277..40311	-	1035	WP_001190234.1	3-deoxy-7-phosphoheptulonate synthase	-
IMP99_RS24575	40871..41179	-	309	WP_000377483.1	hypothetical protein	-
IMP99_RS24580	41278..41460	-	183	WP_001514250.1	hypothetical protein	Toxin
IMP99_RS24585	41457..41654	-	198	WP_001324224.1	toxin-antitoxin system protein	Antitoxin
IMP99_RS24590	42336..43610	+	1275	WP_000489608.1	HlyD family secretion protein	-
IMP99_RS24595	43585..45699	+	2115	WP_001183604.1	peptidase domain-containing ABC transporter	-
IMP99_RS24600	45869..46180	-	312	WP_001259758.1	colicin V	-
IMP99_RS24605	46158..46394	-	237	WP_001323890.1	colicin V immunity protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA5 / blaTEM-1B / sitABCD / aph(6)-Id / aph(3'')-Ib / sul2	iroB / iroC / iroD / iroE / iroN / iucA / iucB / iucC / iucD / iutA	1..150153	150153

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6961.10 Da
• Isoelectric Point: 4.5349

>T176917 WP_001514250.1 NZ_CP062858:c41460-41278 [Escherichia coli O20:H12]
MMEIFWTMLTSQDRKRIREYIAEQNLIAAIELDERIGCSASLLFSLSFISVQVHDNIITV

Nucleotide

• Length: 183 bp

>T176917 NZ_CP083007:4130568-4130786 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7658.53 Da
• Isoelectric Point: 7.2046

>AT176917 WP_001324224.1 NZ_CP062858:c41654-41457 [Escherichia coli O20:H12]
MDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK

Nucleotide

• Length: 198 bp

>AT176917 NZ_CP083007:4130167-4130541 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG