Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3672248..3672866 Replicon chromosome
Accession NZ_CP062855
Organism Escherichia coli O20:H12 strain Res13-Lact-PEB17-18

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag IMP99_RS17880 Protein ID WP_001291435.1
Coordinates 3672648..3672866 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag IMP99_RS17875 Protein ID WP_000344800.1
Coordinates 3672248..3672622 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP99_RS17865 3667337..3668530 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
IMP99_RS17870 3668553..3671702 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
IMP99_RS17875 3672248..3672622 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
IMP99_RS17880 3672648..3672866 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
IMP99_RS17885 3673038..3673589 + 552 WP_000102568.1 maltose O-acetyltransferase -
IMP99_RS17890 3673705..3674175 + 471 WP_000136192.1 YlaC family protein -
IMP99_RS17895 3674339..3675889 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
IMP99_RS17900 3675931..3676284 - 354 WP_000878140.1 DUF1428 domain-containing protein -
IMP99_RS17910 3676663..3676974 + 312 WP_000409911.1 MGMT family protein -
IMP99_RS17915 3677005..3677577 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T176911 WP_001291435.1 NZ_CP062855:3672648-3672866 [Escherichia coli O20:H12]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T176911 NZ_CP083007:325718-326086 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT176911 WP_000344800.1 NZ_CP062855:3672248-3672622 [Escherichia coli O20:H12]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT176911 NZ_CP083007:325500-325721 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References