Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 1024452..1024977 Replicon chromosome
Accession NZ_CP062855
Organism Escherichia coli O20:H12 strain Res13-Lact-PEB17-18

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag IMP99_RS05015 Protein ID WP_001159871.1
Coordinates 1024672..1024977 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag IMP99_RS05010 Protein ID WP_000813630.1
Coordinates 1024452..1024670 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP99_RS04980 1019453..1020043 - 591 WP_000194550.1 hypothetical protein -
IMP99_RS04985 1020043..1020297 - 255 WP_000343103.1 hypothetical protein -
IMP99_RS04990 1020649..1021650 + 1002 WP_000774875.1 DUF4238 domain-containing protein -
IMP99_RS04995 1021826..1022170 - 345 WP_000793307.1 hypothetical protein -
IMP99_RS05000 1022669..1022923 - 255 WP_000678527.1 hypothetical protein -
IMP99_RS05005 1022968..1023894 - 927 WP_001290416.1 hypothetical protein -
IMP99_RS05010 1024452..1024670 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
IMP99_RS05015 1024672..1024977 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
IMP99_RS05020 1024978..1025784 + 807 WP_000016969.1 site-specific integrase -
IMP99_RS05025 1025964..1026608 + 645 WP_001144036.1 ParA family protein -
IMP99_RS05030 1026695..1027003 + 309 WP_000030199.1 hypothetical protein -
IMP99_RS05035 1027417..1028397 + 981 WP_000688514.1 plasmid segregation protein ParM -
IMP99_RS05040 1028390..1028806 + 417 WP_001278818.1 plasmid partitioning/stability family protein -
IMP99_RS05045 1028808..1029254 - 447 Protein_991 DUF4113 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island blaCMY-2 - 1013494..1029140 15646


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T176897 WP_001159871.1 NZ_CP062855:1024672-1024977 [Escherichia coli O20:H12]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T176897 NZ_CP082995:c5250480-5250097 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT176897 WP_000813630.1 NZ_CP062855:1024452-1024670 [Escherichia coli O20:H12]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT176897 NZ_CP082995:c5250722-5250480 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References