Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4163680..4164177 Replicon chromosome
Accession NZ_CP062853
Organism Escherichia coli O9:H10 strain Res13-Lact-PEB20-37-A

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag IMQ02_RS20335 Protein ID WP_001173343.1
Coordinates 4163680..4163853 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag IMQ02_RS20340 Protein ID WP_000398619.1
Coordinates 4163881..4164177 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMQ02_RS20325 4160350..4161807 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
IMQ02_RS20330 4162044..4163561 + 1518 WP_001295074.1 lysine--tRNA ligase -
IMQ02_RS20335 4163680..4163853 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
IMQ02_RS20340 4163881..4164177 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
IMQ02_RS20345 4164404..4164676 - 273 WP_000405647.1 N-acetyltransferase -
IMQ02_RS20350 4164688..4164918 - 231 WP_000371700.1 (4Fe-4S)-binding protein -
IMQ02_RS20355 4165099..4166730 + 1632 WP_001216477.1 sensor histidine kinase -
IMQ02_RS20360 4166727..4167446 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T176891 WP_001173343.1 NZ_CP062853:c4163853-4163680 [Escherichia coli O9:H10]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T176891 NZ_CP082995:1947919-1948021 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT176891 WP_000398619.1 NZ_CP062853:c4164177-4163881 [Escherichia coli O9:H10]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT176891 NZ_CP082995:c1948028-1947883 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References