Detailed information of TA system TA176884

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2390339..2390977
• Replicon: chromosome
• Accession: NZ_CP062853
• Organism: Escherichia coli O9:H10 strain Res13-Lact-PEB20-37-A

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: IMQ02_RS11385
• Protein ID: WP_000813794.1
• Coordinates: 2390801..2390977 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: IMQ02_RS11380
• Protein ID: WP_001270286.1
• Coordinates: 2390339..2390755 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMQ02_RS11360	2385491..2386432	-	942	WP_001251313.1	ABC transporter permease	-
IMQ02_RS11365	2386433..2387446	-	1014	WP_000220399.1	ABC transporter ATP-binding protein	-
IMQ02_RS11370	2387464..2388609	-	1146	WP_000047421.1	ABC transporter substrate-binding protein	-
IMQ02_RS11375	2388854..2390260	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
IMQ02_RS11380	2390339..2390755	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
IMQ02_RS11385	2390801..2390977	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
IMQ02_RS11390	2391199..2391429	+	231	WP_000494244.1	YncJ family protein	-
IMQ02_RS11395	2391521..2393482	-	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
IMQ02_RS11400	2393555..2394091	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
IMQ02_RS11405	2394144..2395358	+	1215	WP_071607224.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2390339..2400670	10331

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T176884 WP_000813794.1 NZ_CP062853:c2390977-2390801 [Escherichia coli O9:H10]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T176884 NZ_CP082989:c14266-13916 [Klebsiella pneumoniae]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT176884 WP_001270286.1 NZ_CP062853:c2390755-2390339 [Escherichia coli O9:H10]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT176884 NZ_CP082989:c13911-13609 [Klebsiella pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• PDB: 6HPB