Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3951600..3952176 Replicon chromosome
Accession NZ_CP062851
Organism Escherichia coli O9:H10 strain Res13-Lact-PEB21-38-A

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag IMQ03_RS19275 Protein ID WP_001297643.1
Coordinates 3951889..3952176 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag IMQ03_RS19270 Protein ID WP_000063160.1
Coordinates 3951600..3951902 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMQ03_RS19255 3948196..3950346 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
IMQ03_RS19260 3950396..3950599 + 204 WP_000467859.1 YbdD/YjiX family protein -
IMQ03_RS19265 3950610..3951566 + 957 WP_195691095.1 GTPase -
IMQ03_RS19270 3951600..3951902 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
IMQ03_RS19275 3951889..3952176 - 288 WP_001297643.1 BrnT family toxin Toxin
IMQ03_RS19280 3952375..3953071 - 697 Protein_3757 IS1 family transposase -
IMQ03_RS19285 3953386..3954618 + 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
IMQ03_RS19290 3954659..3955939 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3952375..3952584 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T176866 WP_001297643.1 NZ_CP062851:c3952176-3951889 [Escherichia coli O9:H10]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T176866 NZ_CP082985:c4757348-4757064 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCTGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT176866 WP_000063160.1 NZ_CP062851:c3951902-3951600 [Escherichia coli O9:H10]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT176866 NZ_CP082985:c4757580-4757338 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References