Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 856617..857271 Replicon chromosome
Accession NZ_CP062851
Organism Escherichia coli O9:H10 strain Res13-Lact-PEB21-38-A

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag IMQ03_RS04160 Protein ID WP_000244777.1
Coordinates 856864..857271 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag IMQ03_RS04155 Protein ID WP_000354046.1
Coordinates 856617..856883 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMQ03_RS04130 851786..852529 + 744 WP_000951948.1 SDR family oxidoreductase -
IMQ03_RS04135 852586..854019 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
IMQ03_RS04140 854064..854375 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
IMQ03_RS04145 854539..855198 + 660 WP_000250274.1 hemolysin III family protein -
IMQ03_RS04150 855394..856374 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
IMQ03_RS04155 856617..856883 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
IMQ03_RS04160 856864..857271 + 408 WP_000244777.1 toxin CptA Toxin
IMQ03_RS04165 857311..857832 - 522 WP_001055874.1 flavodoxin FldB -
IMQ03_RS04170 857944..858840 + 897 WP_000806639.1 site-specific tyrosine recombinase XerD -
IMQ03_RS04175 858865..859575 + 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IMQ03_RS04180 859581..861314 + 1734 WP_195691199.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T176848 WP_000244777.1 NZ_CP062851:856864-857271 [Escherichia coli O9:H10]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T176848 NZ_CP082978:322749-323117 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT176848 WP_000354046.1 NZ_CP062851:856617-856883 [Escherichia coli O9:H10]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT176848 NZ_CP082978:322531-322752 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References