Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 132971..133496 Replicon plasmid unnamednovel_0
Accession NZ_CP062843
Organism Escherichia coli strain Res13-Lact-PER02-33

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag IMQ20_RS24835 Protein ID WP_001159868.1
Coordinates 132971..133276 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag IMQ20_RS24840 Protein ID WP_000813634.1
Coordinates 133278..133496 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMQ20_RS24810 128803..129447 - 645 WP_029488797.1 replication initiation protein RepE -
IMQ20_RS24815 129475..130380 - 906 Protein_142 IS3 family transposase -
IMQ20_RS24820 130445..131596 + 1152 WP_195686658.1 IS30-like element IS30 family transposase -
IMQ20_RS24825 131609..131911 - 303 Protein_144 transposase -
IMQ20_RS24830 132164..132970 - 807 WP_097423266.1 site-specific integrase -
IMQ20_RS24835 132971..133276 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
IMQ20_RS24840 133278..133496 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
IMQ20_RS24845 134210..136831 + 2622 WP_023063774.1 ABC transporter -
IMQ20_RS24850 137030..137260 + 231 WP_029488776.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
IMQ20_RS24855 137349..137606 + 258 WP_195686660.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..141262 141262
- flank IS/Tn - - 137762..138970 1208


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T176844 WP_001159868.1 NZ_CP062843:c133276-132971 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T176844 NZ_CP082975:c5233061-5232678 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT176844 WP_000813634.1 NZ_CP062843:c133496-133278 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT176844 NZ_CP082975:c5233303-5233061 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTTCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References