Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 105973..106574 Replicon plasmid unnamed1009
Accession NZ_CP062839
Organism Escherichia coli strain Res13-Lact-PER02-33

Toxin (Protein)


Gene name doc Uniprot ID U9Z1Q0
Locus tag IMQ20_RS23330 Protein ID WP_001216047.1
Coordinates 105973..106353 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag IMQ20_RS23335 Protein ID WP_001190712.1
Coordinates 106353..106574 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMQ20_RS23305 101414..102898 - 1485 WP_000124159.1 hypothetical protein -
IMQ20_RS23310 102898..104091 - 1194 WP_047649469.1 hypothetical protein -
IMQ20_RS23315 104177..104629 - 453 WP_001326849.1 late promoter-activating protein -
IMQ20_RS23320 104718..105761 - 1044 WP_195686630.1 DUF968 domain-containing protein -
IMQ20_RS23325 105789..105968 - 180 WP_000113018.1 hypothetical protein -
IMQ20_RS23330 105973..106353 - 381 WP_001216047.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
IMQ20_RS23335 106353..106574 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
IMQ20_RS23340 106647..107036 - 390 WP_000506730.1 DNA repair protein -
IMQ20_RS23345 107211..107795 + 585 WP_042198310.1 hypothetical protein -
IMQ20_RS23350 107796..108152 + 357 WP_001062545.1 hypothetical protein -
IMQ20_RS23355 109228..109590 - 363 WP_001261543.1 hypothetical protein -
IMQ20_RS23360 109587..110597 - 1011 WP_042198315.1 hypothetical protein -
IMQ20_RS23365 110931..111109 - 179 Protein_115 hypothetical protein -
IMQ20_RS23370 111111..111299 - 189 WP_000797279.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid ant(3'')-Ia / aac(3)-VIa / sul1 htpB 1..118942 118942


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13574.27 Da        Isoelectric Point: 5.1514

>T176832 WP_001216047.1 NZ_CP062839:c106353-105973 [Escherichia coli]
MRHISPEELVALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T176832 NZ_CP082967:c5382540-5382157 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT176832 WP_001190712.1 NZ_CP062839:c106574-106353 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT176832 NZ_CP082967:c5382782-5382540 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9Z1Q0

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References