Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 132964..133489 Replicon plasmid unnamednovel_0
Accession NZ_CP062837
Organism Escherichia coli strain Res13-Lact-PER04-33

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag IMQ22_RS24785 Protein ID WP_001159868.1
Coordinates 132964..133269 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag IMQ22_RS24790 Protein ID WP_000813634.1
Coordinates 133271..133489 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMQ22_RS24760 128796..129440 - 645 WP_029488797.1 replication initiation protein RepE -
IMQ22_RS24765 129468..130373 - 906 Protein_142 IS3 family transposase -
IMQ22_RS24770 130438..131589 + 1152 WP_195686658.1 IS30-like element IS30 family transposase -
IMQ22_RS24775 131602..131904 - 303 Protein_144 transposase -
IMQ22_RS24780 132157..132963 - 807 WP_097423266.1 site-specific integrase -
IMQ22_RS24785 132964..133269 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
IMQ22_RS24790 133271..133489 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
IMQ22_RS24795 134203..136824 + 2622 WP_023063774.1 ABC transporter -
IMQ22_RS24800 137023..137253 + 231 WP_029488776.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
IMQ22_RS24805 137342..137599 + 258 WP_195686660.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..141255 141255
- flank IS/Tn - - 137755..138963 1208


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T176808 WP_001159868.1 NZ_CP062837:c133269-132964 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T176808 NZ_CP082954:897059-897472 [Salmonella enterica subsp. arizonae]
GTGGTCCTGTGGCAATCTGAATTACGCGTCTCATGGCGCGCTCAGTGGATCTCGTTGCTTATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCATTGTGGATGATGCTACTGTCGCTGGTCGTGTTTGACTGCG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAAATCAAGCTGCTAATGGATGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTTTTGCGTCCGCCCTGGTTACTGAGGAGCGGGATGGTGTTGCGTTTACGCGCTGAGTCTGGGCACCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGCAGCGGAATGGCGTGAGTTGCGTCGGATAATGTTACAGCAACCAATAT
CGCGCCAACACTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT176808 WP_000813634.1 NZ_CP062837:c133489-133271 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT176808 NZ_CP082954:896812-897078 [Salmonella enterica subsp. arizonae]
ATGGACATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGCGACGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTGATGAATCACGGTAAACCGGTTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References