Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4269144..4269641 Replicon chromosome
Accession NZ_CP062832
Organism Escherichia coli strain Res13-Lact-PER04-33

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag IMQ22_RS20600 Protein ID WP_001173343.1
Coordinates 4269144..4269317 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag IMQ22_RS20605 Protein ID WP_000398619.1
Coordinates 4269345..4269641 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMQ22_RS20590 4265814..4267271 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
IMQ22_RS20595 4267508..4269025 + 1518 WP_001295074.1 lysine--tRNA ligase -
IMQ22_RS20600 4269144..4269317 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
IMQ22_RS20605 4269345..4269641 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
IMQ22_RS20610 4269868..4270140 - 273 WP_000405651.1 N-acetyltransferase -
IMQ22_RS20615 4270152..4270382 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
IMQ22_RS20620 4270563..4272194 + 1632 WP_001216477.1 sensor histidine kinase -
IMQ22_RS20625 4272191..4272910 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T176795 WP_001173343.1 NZ_CP062832:c4269317-4269144 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T176795 NZ_CP082948:c977737-977471 [Streptococcus suis]
TTGAGTTATAAGGTTATTTTGTCTGCTAAGGCTCAAAAGCAAATTCGAAAGATGGATAAAACGGCTGTAAGTTTGATTTT
GCGGTATCTTTACAAGCATATTGACGGGTGCGACAATCCCAGACAGTACGGTAAAGCCTTGACGGCCAATCGTTCTGGTC
AATGGCGCTATCGTATCGGCAACTACAGGGTTATCGTCAGCATTGAAGATGACAAATTGATTGTGACAGCCCTTGAAGTC
GGGCATCGGAAAGAAATATATAAATAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT176795 WP_000398619.1 NZ_CP062832:c4269641-4269345 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT176795 NZ_CP082948:c977966-977727 [Streptococcus suis]
ATGGCAGCTATCACATTGAAAGTTTCTGAGCAGGACAAGCTCTTTATGCAGGCTATGGCAAAGTTTGAAGGTGTCAGTTT
GTCAGAACTCATTCGGACTAAAACCCTTGAAGCCCTTGAAGACGAATACGATGCGCGTGTGGCAGACCTTGCTTGGGCAG
AATACCAAGAAGACTTGGCGAATGGCATTGAGCCGATAACATTAGAACAAATGGCTGAGGAGTTGGGCATTGAGTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References