Detailed information of TA system TA176779

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1049690..1050273
• Replicon: chromosome
• Accession: NZ_CP062832
• Organism: Escherichia coli strain Res13-Lact-PER04-33

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IMQ22_RS05030
• Protein ID: WP_113263827.1
• Coordinates: 1049938..1050273 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A1V2T5M9
• Locus tag: IMQ22_RS05025
• Protein ID: WP_000581941.1
• Coordinates: 1049690..1049938 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMQ22_RS05015	1046029..1047330	+	1302	WP_000046812.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-
IMQ22_RS05020	1047378..1049612	+	2235	WP_000226815.1	GTP pyrophosphokinase	-
IMQ22_RS05025	1049690..1049938	+	249	WP_000581941.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IMQ22_RS05030	1049938..1050273	+	336	WP_113263827.1	endoribonuclease MazF	Toxin
IMQ22_RS05035	1050344..1051135	+	792	WP_001071648.1	nucleoside triphosphate pyrophosphohydrolase	-
IMQ22_RS05040	1051363..1053000	+	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
IMQ22_RS05045	1053088..1054386	+	1299	WP_000036723.1	phosphopyruvate hydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12141.07 Da
• Isoelectric Point: 8.7218

>T176779 WP_113263827.1 NZ_CP062832:1049938-1050273 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQRIKAKINVLIG

Nucleotide

• Length: 336 bp

>T176779 NZ_CP082930:155309-155614 [Salmonella enterica subsp. enterica serovar London]
ATGCAGTTTAAGGTTTACACCTATAAAAAGGAGAGTCGCTACACCCTGTTCGTGGATGTCCAGAGTGACATTATCGACAC
CCCCGGACGGCGGATGGTGATCCCGCTGGCCAGCGCCCGTCTGCTGTCGGATAAGGTCTCCCGCGAACTTTATCCTGTGG
TACATATAGGGGATGAGAGCTGGCGTCTGATGACCACAGATATGGCCAGTGTGCCGGCCTCAGTCATCGGGGAAGAGGTG
GCCGATCTCAGTCACCGGGAGAACGATATCAAAAACGCCATTAACCTGATGTTCTGGGGGATTTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9385.76 Da
• Isoelectric Point: 4.4665

>AT176779 WP_000581941.1 NZ_CP062832:1049690-1049938 [Escherichia coli]
MIHSSVKRWGNSPAVRIPTTLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT176779 NZ_CP082930:155089-155307 [Salmonella enterica subsp. enterica serovar London]
ATGAAGCAGCGTATTACAGTGACAGTGGACAGCGACAGCTATCAGCTGCTCAAGGCATACGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGCCGTCTGCGAGCCGAACGCTGGCAGGAAGAAAACCGGGAAGGTATGGCTG
AGGTGGCCAGCTTTATAGAGGCTAACGGATCGTTTGCTGACGAAAACAGGAACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1V2T5M9