Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 132971..133496 Replicon plasmid unnamednovel_0
Accession NZ_CP062831
Organism Escherichia coli strain Res13-Lact-PER12-33-A

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag IMQ30_RS24795 Protein ID WP_001159868.1
Coordinates 132971..133276 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag IMQ30_RS24800 Protein ID WP_000813634.1
Coordinates 133278..133496 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMQ30_RS24770 128803..129447 - 645 WP_029488797.1 replication initiation protein RepE -
IMQ30_RS24775 129475..130380 - 906 Protein_141 IS3 family transposase -
IMQ30_RS24780 130445..131596 + 1152 WP_195686658.1 IS30-like element IS30 family transposase -
IMQ30_RS24785 131609..131911 - 303 Protein_143 transposase -
IMQ30_RS24790 132164..132970 - 807 WP_097423266.1 site-specific integrase -
IMQ30_RS24795 132971..133276 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
IMQ30_RS24800 133278..133496 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
IMQ30_RS24805 134210..136831 + 2622 WP_023063774.1 ABC transporter -
IMQ30_RS24810 137030..137260 + 231 WP_029488776.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
IMQ30_RS24815 137349..137606 + 258 WP_195686660.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..141262 141262
- flank IS/Tn - - 137762..138970 1208


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T176772 WP_001159868.1 NZ_CP062831:c133276-132971 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T176772 NZ_CP082929:c4055120-4054836 [Salmonella enterica subsp. enterica serovar London]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGAAGTTGTGATTGTGTTTGTTGTCGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT176772 WP_000813634.1 NZ_CP062831:c133496-133278 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT176772 NZ_CP082929:c4055352-4055110 [Salmonella enterica subsp. enterica serovar London]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References