Detailed information of TA system TA176766

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 75372..75919
• Replicon: plasmid unnamednovel_0
• Accession: NZ_CP062831
• Organism: Escherichia coli strain Res13-Lact-PER12-33-A

Toxin (Protein)

• Gene name: parE
• Uniprot ID: E9LLH3
• Locus tag: IMQ30_RS24465
• Protein ID: WP_000969996.1
• Coordinates: 75372..75653 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A2H4TKU7
• Locus tag: IMQ30_RS24470
• Protein ID: WP_000079923.1
• Coordinates: 75650..75919 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMQ30_RS24440	71587..72588	+	1002	WP_029488840.1	DUF2219 family protein	-
IMQ30_RS24445	72663..73568	-	906	Protein_75	IS3 family transposase	-
IMQ30_RS24450	73578..74729	-	1152	WP_195686650.1	IS30-like element IS30 family transposase	-
IMQ30_RS24455	74797..75099	-	303	Protein_77	transposase	-
IMQ30_RS24460	75135..75332	-	198	Protein_78	3'-5' exonuclease	-
IMQ30_RS24465	75372..75653	-	282	WP_000969996.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IMQ30_RS24470	75650..75919	-	270	WP_000079923.1	hypothetical protein	Antitoxin
IMQ30_RS24475	76838..77694	-	857	Protein_81	incFII family plasmid replication initiator RepA	-
IMQ30_RS24480	77687..77761	-	75	WP_001365705.1	RepA leader peptide Tap	-
IMQ30_RS24485	78008..78256	-	249	WP_000083838.1	replication regulatory protein RepA	-
IMQ30_RS24490	78540..78746	-	207	WP_032339099.1	type I toxin-antitoxin system Hok family toxin	-
-	78733..78794	+	62	NuclAT_0	-	-
-	78733..78794	+	62	NuclAT_0	-	-
-	78733..78794	+	62	NuclAT_0	-	-
-	78733..78794	+	62	NuclAT_0	-	-
IMQ30_RS24495	78969..79352	-	384	Protein_85	hypothetical protein	-
IMQ30_RS24500	79470..79916	-	447	WP_032339098.1	thermonuclease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..141262	141262
	-	inside	IScluster/Tn	-	-	72663..86490	13827

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10893.57 Da
• Isoelectric Point: 8.4615

>T176766 WP_000969996.1 NZ_CP062831:c75653-75372 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T176766 NZ_CP082929:842078-842476 [Salmonella enterica subsp. enterica serovar London]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCCGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAGTTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10196.52 Da
• Isoelectric Point: 7.2050

>AT176766 WP_000079923.1 NZ_CP062831:c75919-75650 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVEQQHNTWFRDQVAAGRQQLERGDVLPHDMVESSATAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT176766 NZ_CP082929:841851-842078 [Salmonella enterica subsp. enterica serovar London]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGTAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTCTGA

Structures

Toxin

• AlphaFold DB: E9LLH3

Antitoxin

• AlphaFold DB: A0A2H4TKU7