Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3979714..3980290 Replicon chromosome
Accession NZ_CP062826
Organism Escherichia coli strain Res13-Lact-PER12-33-A

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag IMQ30_RS19215 Protein ID WP_001297643.1
Coordinates 3980003..3980290 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag IMQ30_RS19210 Protein ID WP_000063160.1
Coordinates 3979714..3980016 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMQ30_RS19195 3976310..3978460 + 2151 WP_001340937.1 pyruvate/proton symporter BtsT -
IMQ30_RS19200 3978510..3978713 + 204 WP_000467859.1 YbdD/YjiX family protein -
IMQ30_RS19205 3978724..3979680 + 957 WP_001297640.1 GTPase -
IMQ30_RS19210 3979714..3980016 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
IMQ30_RS19215 3980003..3980290 - 288 WP_001297643.1 BrnT family toxin Toxin
IMQ30_RS19220 3980489..3981185 - 697 Protein_3757 IS1 family transposase -
IMQ30_RS19225 3981500..3982732 + 1233 WP_001137031.1 multidrug efflux MFS transporter MdtM -
IMQ30_RS19230 3982773..3984053 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3980489..3980698 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T176757 WP_001297643.1 NZ_CP062826:c3980290-3980003 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T176757 NZ_CP082926:4188405-4188698 [Shewanella inventionis]
ATGTCGAAATTCGAATTATCAGGTAAAGCTAAAAGCGATTTAATAAAAGTAGCCAAGTATACTCAGTTAACTTGGGGAAC
AGCACAGCGAAATGATTATTTAAAACTGTTAGATAATACTTTCCATCAATTAGCCGATGAACCTATGCTGGGAGTTAATT
GTGACTATATAAGTGAAGGTTATCGAAAACAGCCTCAGGGTAGTCACATAATTTATTATAAAGAACATAAAACAAACCAA
ATTTTAATTGTAAGAATATTACACAAAAGTATGGATGTTAACCGCGCTCTCTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT176757 WP_000063160.1 NZ_CP062826:c3980016-3979714 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT176757 NZ_CP082926:4188166-4188405 [Shewanella inventionis]
ATGGCTAAGAATACAAGCGTAACATTAGGCGAACATTTTGATCAATTTATAAATCAGCAACTTAAATCAGGTCGTTATGG
CTCAGCAAGTGAAGTTGTGCGTGCTGGTTTACGAGTTTTAGAAGATCAAGAAACAAAGATAATGAATTTACGCAATATGC
TAACTGAAGGTGAAAACAGTGGCGTTACAGATTACTCGTATGATTCTTTGATTTCAGAGCTGGACCAAGATAGCCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References