Detailed information of TA system TA176754

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2364527..2366115
• Replicon: chromosome
• Accession: NZ_CP062826
• Organism: Escherichia coli strain Res13-Lact-PER12-33-A

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: S1ELM3
• Locus tag: IMQ30_RS11490
• Protein ID: WP_001125439.1
• Coordinates: 2364793..2366115 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: IMQ30_RS11485
• Protein ID: WP_001301023.1
• Coordinates: 2364527..2364793 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMQ30_RS11480	2360720..2364304	+	3585	Protein_2245	autotransporter barrel domain-containing lipoprotein	-
IMQ30_RS11485	2364527..2364793	+	267	WP_001301023.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
IMQ30_RS11490	2364793..2366115	+	1323	WP_001125439.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
IMQ30_RS11495	2366427..2366635	+	209	Protein_2248	helix-turn-helix transcriptional regulator	-
IMQ30_RS11500	2366890..2368116	+	1227	Protein_2249	fimbrial biogenesis outer membrane usher protein	-
IMQ30_RS11505	2368130..2368660	+	531	WP_000876763.1	type 1 fimbrial protein	-
IMQ30_RS11510	2368673..2369176	+	504	WP_000825452.1	type 1 fimbrial protein	-
IMQ30_RS11515	2369235..2370149	+	915	WP_000520676.1	fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2357548..2377335	19787

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49275.82 Da
• Isoelectric Point: 8.2482

>T176754 WP_001125439.1 NZ_CP062826:2364793-2366115 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T176754 NZ_CP082926:3944552-3944866 [Shewanella inventionis]
ATGCACAATGAGACAGTGCAAGTTGCAATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAA
ACGACATCGTTTAATTCCAAGTAGCATAGAGAATGCTCTATTTCGGAAGCTAGAAATCTTAGATGCAGCTCAAGCTGAAT
CAGACTTAAGAATTCCACCGGGTAATCGTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAAC
AAACAGTATCGTTTGATTTTTCAGTGGGTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATCAGTATTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.38 Da
• Isoelectric Point: 6.2267

>AT176754 WP_001301023.1 NZ_CP062826:2364527-2364793 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Nucleotide

• Length: 267 bp

>AT176754 NZ_CP082926:3944877-3945194 [Shewanella inventionis]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCTATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTGAGTAATTTGATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCAGCGGCATTAGGCAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCTTTATCAAGAAGAAGCAAAGTTTGTAAAACCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Structures

Toxin

• PDB: 5K98
• PDB: 3TPV
• PDB: 3DNV
• PDB: 3TPD
• PDB: 3HZI
• PDB: 3FBR
• PDB: 4YG7
• PDB: 3TPE
• PDB: 2WIU
• PDB: 3DNT
• PDB: 3TPB
• PDB: 3TPT
• PDB: 3DNU