Detailed information of TA system TA176743

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1049685..1050268
• Replicon: chromosome
• Accession: NZ_CP062826
• Organism: Escherichia coli strain Res13-Lact-PER12-33-A

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IMQ30_RS05030
• Protein ID: WP_113263827.1
• Coordinates: 1049933..1050268 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A1V2T5M9
• Locus tag: IMQ30_RS05025
• Protein ID: WP_000581941.1
• Coordinates: 1049685..1049933 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMQ30_RS05015	1046024..1047325	+	1302	WP_000046812.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-
IMQ30_RS05020	1047373..1049607	+	2235	WP_000226815.1	GTP pyrophosphokinase	-
IMQ30_RS05025	1049685..1049933	+	249	WP_000581941.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IMQ30_RS05030	1049933..1050268	+	336	WP_113263827.1	endoribonuclease MazF	Toxin
IMQ30_RS05035	1050339..1051130	+	792	WP_001071648.1	nucleoside triphosphate pyrophosphohydrolase	-
IMQ30_RS05040	1051358..1052995	+	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
IMQ30_RS05045	1053083..1054381	+	1299	WP_000036723.1	phosphopyruvate hydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12141.07 Da
• Isoelectric Point: 8.7218

>T176743 WP_113263827.1 NZ_CP062826:1049933-1050268 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQRIKAKINVLIG

Nucleotide

• Length: 336 bp

>T176743 NZ_CP082924:518538-518888 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9385.76 Da
• Isoelectric Point: 4.4665

>AT176743 WP_000581941.1 NZ_CP062826:1049685-1049933 [Escherichia coli]
MIHSSVKRWGNSPAVRIPTTLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT176743 NZ_CP082924:518252-518533 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Antitoxin

• AlphaFold DB: A0A1V2T5M9