Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2575504..2576001 Replicon chromosome
Accession NZ_CP062824
Organism Escherichia coli O167:H26 strain Res13-Lact-PER13-34

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag IMQ31_RS12300 Protein ID WP_001173343.1
Coordinates 2575504..2575677 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag IMQ31_RS12305 Protein ID WP_000398619.1
Coordinates 2575705..2576001 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMQ31_RS12290 2572174..2573631 + 1458 WP_000856814.1 dipeptide/tripeptide permease DtpC -
IMQ31_RS12295 2573868..2575385 + 1518 WP_000003806.1 lysine--tRNA ligase -
IMQ31_RS12300 2575504..2575677 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
IMQ31_RS12305 2575705..2576001 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
IMQ31_RS12310 2576231..2576503 - 273 WP_000405647.1 N-acetyltransferase -
IMQ31_RS12315 2576515..2576745 - 231 WP_085458424.1 (4Fe-4S)-binding protein -
IMQ31_RS12320 2576926..2578557 + 1632 WP_001216477.1 sensor histidine kinase -
IMQ31_RS12325 2578554..2579273 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T176726 WP_001173343.1 NZ_CP062824:c2575677-2575504 [Escherichia coli O167:H26]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T176726 NZ_CP082916:3101305-3101703 [Salmonella enterica subsp. enterica serovar Newlands]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT176726 WP_000398619.1 NZ_CP062824:c2576001-2575705 [Escherichia coli O167:H26]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT176726 NZ_CP082916:3101078-3101305 [Salmonella enterica subsp. enterica serovar Newlands]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGTAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References