Detailed information of TA system TA176718

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 644732..646320
• Replicon: chromosome
• Accession: NZ_CP062824
• Organism: Escherichia coli O167:H26 strain Res13-Lact-PER13-34

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: U9YVT8
• Locus tag: IMQ31_RS03300
• Protein ID: WP_001125415.1
• Coordinates: 644998..646320 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: IMQ31_RS03295
• Protein ID: WP_001296726.1
• Coordinates: 644732..644998 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMQ31_RS03295	644732..644998	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
IMQ31_RS03300	644998..646320	+	1323	WP_001125415.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
IMQ31_RS03305	646503..647177	-	675	Protein_649	LysR family transcriptional regulator	-
IMQ31_RS03310	647317..647625	+	309	WP_001221046.1	hypothetical protein	-
IMQ31_RS03315	647634..648323	+	690	WP_000801314.1	type 1 glutamine amidotransferase domain-containing protein	-
IMQ31_RS03320	648385..649332	+	948	WP_085458756.1	aldo/keto reductase	-
IMQ31_RS03325	649435..649590	+	156	WP_001327998.1	hypothetical protein	-
IMQ31_RS03330	649550..649969	+	420	WP_001314733.1	amidohydrolase family protein	-
IMQ31_RS03335	649920..650111	+	192	WP_001306054.1	amidohydrolase family protein	-
IMQ31_RS03340	650261..650833	+	573	WP_000789914.1	NAD(P)H-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49384.05 Da
• Isoelectric Point: 8.5830

>T176718 WP_001125415.1 NZ_CP062824:644998-646320 [Escherichia coli O167:H26]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHRRLSREYGIK

Nucleotide

• Length: 1323 bp

>T176718 NZ_CP082916:c1657893-1657609 [Salmonella enterica subsp. enterica serovar Newlands]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGCAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGCTTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGTAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT176718 WP_001296726.1 NZ_CP062824:644732-644998 [Escherichia coli O167:H26]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT176718 NZ_CP082916:c1658125-1657883 [Salmonella enterica subsp. enterica serovar Newlands]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: U9YVT8