Detailed information of TA system TA176717

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 563227..563753
• Replicon: chromosome
• Accession: NZ_CP062824
• Organism: Escherichia coli O167:H26 strain Res13-Lact-PER13-34

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: IMQ31_RS02855
• Protein ID: WP_000323025.1
• Coordinates: 563466..563753 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: IMQ31_RS02850
• Protein ID: WP_000534858.1
• Coordinates: 563227..563466 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMQ31_RS02825	558489..559103	+	615	WP_001265627.1	hypothetical protein	-
IMQ31_RS02830	559106..560128	+	1023	WP_195714904.1	DUF4868 domain-containing protein	-
IMQ31_RS02835	560612..561925	+	1314	WP_000589005.1	ISNCY family transposase	-
IMQ31_RS02840	562362..562694	-	333	WP_001301033.1	protein FlxA	-
IMQ31_RS02845	562897..563202	-	306	WP_001326990.1	hypothetical protein	-
IMQ31_RS02850	563227..563466	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
IMQ31_RS02855	563466..563753	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
IMQ31_RS02860	563825..563980	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
IMQ31_RS02865	564197..564448	+	252	WP_000980994.1	hypothetical protein	-
IMQ31_RS02870	564515..564793	+	279	WP_012304870.1	hypothetical protein	-
IMQ31_RS02875	564795..565844	+	1050	WP_195714905.1	DUF968 domain-containing protein	-
IMQ31_RS02880	565858..566610	+	753	WP_001047135.1	antitermination protein	-
IMQ31_RS02885	566888..566977	-	90	WP_120795389.1	hypothetical protein	-
IMQ31_RS02890	567032..567244	-	213	WP_000087756.1	cold shock-like protein CspF	-
IMQ31_RS02895	567545..567760	+	216	WP_000066484.1	cold shock-like protein CspB	-
IMQ31_RS02900	568514..568729	+	216	WP_000839590.1	phage lysis protein EssD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	537084..599153	62069

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T176717 WP_000323025.1 NZ_CP062824:563466-563753 [Escherichia coli O167:H26]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T176717 NZ_CP082916:c1616792-1616484 [Salmonella enterica subsp. enterica serovar Newlands]
ATGCAATACATGGTTTATCGCAATAAAGGAAACAGCAAGGCTTATCCTTATTTACTTGATGTGCAAAGCGATATTATTGA
TGAACTGCATACTCGCATGGTTATCCCATTGTTTCCTGTGAGCAGATTGGTAAATAACCCAGTTAAGCGCCTCACGCCCA
CTCTGAATGTGGAAGGTAATGACTATCTGGTGATGACTCACGAAATGGCGAGTATCAGGCTTTCGCAGATTGGTGATGAG
GTGATGGACGTTCGTTCCCACAGGCAAACCATCAAAAATGCTCTCGATTTTATCTTTGACGGGTTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT176717 WP_000534858.1 NZ_CP062824:563227-563466 [Escherichia coli O167:H26]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT176717 NZ_CP082916:c1617034-1616795 [Salmonella enterica subsp. enterica serovar Newlands]
ATGCCGGGTATGAGTGTACGACATAAAAAAACAGTCAGTGTCACGCTGGAACCTGCATTGCTTCAGCAAGCCCGTGATGC
AGGTATTAATCTGTCAGCGGTGCTTACCGCCGCGCTGAAAGAGGAAATCAGTGCCACAGGCGTTGAACGCTGGAAAGCAG
AAAATAGAGCGGGTTTGCAGGAGCTCAATCGTATTACTGATGAGCACGGCCTTTTATCGGACGATTACAGGACGTTTTAA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8