Detailed information of TA system TA176692

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 75372..75919
• Replicon: plasmid unnamednovel_0
• Accession: NZ_CP062820
• Organism: Escherichia coli strain Res13-Sevr-PER06-05-b-A

Toxin (Protein)

• Gene name: parE
• Uniprot ID: E9LLH3
• Locus tag: IMQ58_RS24490
• Protein ID: WP_000969996.1
• Coordinates: 75372..75653 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A2H4TKU7
• Locus tag: IMQ58_RS24495
• Protein ID: WP_000079923.1
• Coordinates: 75650..75919 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMQ58_RS24460	70899..71090	-	192	WP_157895655.1	hypothetical protein	-
IMQ58_RS24465	71587..72588	+	1002	WP_029488840.1	DUF2219 family protein	-
IMQ58_RS24470	72663..73568	-	906	Protein_77	IS3 family transposase	-
IMQ58_RS24475	73578..74729	-	1152	WP_195706813.1	IS30 family transposase	-
IMQ58_RS24480	74797..75099	-	303	Protein_79	transposase	-
IMQ58_RS24485	75135..75332	-	198	Protein_80	3'-5' exonuclease	-
IMQ58_RS24490	75372..75653	-	282	WP_000969996.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IMQ58_RS24495	75650..75919	-	270	WP_000079923.1	hypothetical protein	Antitoxin
IMQ58_RS24500	76835..77691	-	857	Protein_83	incFII family plasmid replication initiator RepA	-
IMQ58_RS24505	77684..77758	-	75	WP_001365705.1	RepA leader peptide Tap	-
IMQ58_RS24510	78005..78253	-	249	WP_000083838.1	replication regulatory protein RepA	-
IMQ58_RS24515	78537..78743	-	207	WP_032339099.1	type I toxin-antitoxin system Hok family toxin	-
-	78730..78791	+	62	NuclAT_0	-	-
-	78730..78791	+	62	NuclAT_0	-	-
-	78730..78791	+	62	NuclAT_0	-	-
-	78730..78791	+	62	NuclAT_0	-	-
IMQ58_RS24520	78966..79349	-	384	Protein_87	hypothetical protein	-
IMQ58_RS24525	79467..79913	-	447	WP_032339098.1	thermonuclease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..141259	141259
	-	inside	IScluster/Tn	-	-	72663..86487	13824

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10893.57 Da
• Isoelectric Point: 8.4615

>T176692 WP_000969996.1 NZ_CP062820:c75653-75372 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T176692 NZ_CP082909:1673457-1673873 [Endozoicomonas sp. 4G]
ATGAAATACTTACTTGACACCAATGTACTGATTCACATTCTGAGAGGACACGACAAAGGTAGTATCAAAGAGCATTTCAA
AAGCAACAATGGCCGTATGTTTATATCGGCGGTTACTGTTGATGAGCTTATGTTTGGCATTTGCTGCGCCCCAAAAAGAC
TTCGGGAGAAAAAACAGGAAGAGTTAAATACTCTCCTTAGTGTTATCCCGATACTAGATTTTAACGAGGATGCAGCGGAG
CACTCTTCTCAAATTCGATACTTTCTGAAATCTACTGGTCAGCTGATTGGTAGCTATGACAATATGATAGCTGGGCATGC
CCGAAGTCTGGAATTGACCGTGATCACCAATAACACTAAAGAATTTTGTAGAGTGCCGGGTTTAGAGGTTGAAGACTGGT
CGCAACCCTTGACATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10196.52 Da
• Isoelectric Point: 7.2050

>AT176692 WP_000079923.1 NZ_CP062820:c75919-75650 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVEQQHNTWFRDQVAAGRQQLERGDVLPHDMVESSATAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT176692 NZ_CP082909:1673224-1673457 [Endozoicomonas sp. 4G]
ATGAAAACGACGATTTTCAAAAACAACAGAACGCAGGCTGTACGCATTCCCAAAGAGTTTGCGTTTCCCGAAAATGTAAA
GCACGTTCAAATCCAGCGACAAGGGAATACTTTAACCATTTCACCCGTAGATGTGGACTGGGACGAATTTTTTGACAACA
TCACGGTCTGTCCGGATTACCCTGAGAGAGAACAGCCTCTACCACAAGAACGTGATTTTGGAGATTGGTCATAA

Structures

Toxin

• AlphaFold DB: E9LLH3

Antitoxin

• AlphaFold DB: A0A2H4TKU7