Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3979189..3979765 Replicon chromosome
Accession NZ_CP062815
Organism Escherichia coli strain Res13-Sevr-PER06-05-b-A

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag IMQ58_RS19230 Protein ID WP_001297643.1
Coordinates 3979478..3979765 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag IMQ58_RS19225 Protein ID WP_000063160.1
Coordinates 3979189..3979491 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMQ58_RS19210 3975785..3977935 + 2151 WP_001340937.1 pyruvate/proton symporter BtsT -
IMQ58_RS19215 3977985..3978188 + 204 WP_000467859.1 YbdD/YjiX family protein -
IMQ58_RS19220 3978199..3979155 + 957 WP_001297640.1 GTPase -
IMQ58_RS19225 3979189..3979491 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
IMQ58_RS19230 3979478..3979765 - 288 WP_001297643.1 BrnT family toxin Toxin
IMQ58_RS19235 3979964..3980660 - 697 Protein_3760 IS1 family transposase -
IMQ58_RS19240 3980975..3982207 + 1233 WP_001137031.1 multidrug efflux MFS transporter MdtM -
IMQ58_RS19245 3982248..3983528 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3979964..3980173 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T176683 WP_001297643.1 NZ_CP062815:c3979765-3979478 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T176683 NZ_CP082909:148988-149434 [Endozoicomonas sp. 4G]
GTGAAGTATTTACTGGATACCTGCGTGTTGTCAGAAGCATTCAAAAAAAATCCTGATAAGCATGTACTTGAATGGATCGA
TAGTCGTGAGGAAAACAGGCTTTATTTGAGTGTTTTAACTTTGGCAGAAATTGAAAAGGGAATAATAAAATGCCAGGAAC
CGGTAAGGCAGGAAAAGCTGCGAACCTGGCTTGAGTCTGATTTAATGGAGCGCTTCAAGCACAGAATATTACCTATAGAT
CTTGGGGTGGCAATAGCCTGGGGGCGTATTCAGGCTGAATCTGAAAAAAAGGGTACACCTATGCCCGTTATTGATGGGCT
TATAGCTGTCACAGGATTAGTGAATGACTGTACTGTAGTTACTCGAAATACAGGCGATATGCGCATGAGTCAGGTTCAGT
TATATAACCCTTGGGAAGACACGGCACCTTTCTGCGGTGTCAATTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT176683 WP_000063160.1 NZ_CP062815:c3979491-3979189 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT176683 NZ_CP082909:148719-148991 [Endozoicomonas sp. 4G]
ATGACTGAGGGGATTAGTATGGAAAATCAATGGCAGTTACAGGAGGCGAAAAGCAAGTTCAGTCATATTGTGGACAGGGC
TCGTCAGCATGGTCCACAATTCGTGACCCGTCATGGGCATAAAGCGGCTGTCGTCCTCTCATTTGAAGACTATCAAAGGA
TGTGTGCACCAGAGAATAGTCTGAGTGAGTTTCTTAAAGCCTCACCCTTTTCAGATTCTGATGTTGAAACGTTTGACAGA
AGTAAAGAAACCCCGAGGGATGTAGATCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References