Detailed information of TA system TA176653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4128956..4129506
• Replicon: chromosome
• Accession: NZ_CP062811
• Organism: Escherichia coli strain Res13-Sevr-PER07-33

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: IMQ59_RS19670
• Protein ID: WP_001160975.1
• Coordinates: 4128956..4129270 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: D3H431
• Locus tag: IMQ59_RS19675
• Protein ID: WP_000125565.1
• Coordinates: 4129273..4129506 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMQ59_RS19645	4124631..4125917	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
IMQ59_RS19650	4125917..4126906	+	990	WP_000241272.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
IMQ59_RS19655	4126903..4127724	+	822	WP_001065381.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
IMQ59_RS19660	4127727..4128104	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
IMQ59_RS19665	4128111..4128959	+	849	WP_000257185.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
IMQ59_RS19670	4128956..4129270	-	315	WP_001160975.1	CcdB family protein	Toxin
IMQ59_RS19675	4129273..4129506	-	234	WP_000125565.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
IMQ59_RS19680	4129592..4130071	-	480	WP_001487721.1	type 3 dihydrofolate reductase	-
IMQ59_RS19685	4130263..4132125	-	1863	WP_021574448.1	glutathione-regulated potassium-efflux system protein KefC	-
IMQ59_RS19690	4132118..4132648	-	531	WP_000600718.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
IMQ59_RS19695	4132759..4134090	-	1332	WP_001183207.1	MFS transporter	-
IMQ59_RS19700	4134149..4134436	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T176653 WP_001160975.1 NZ_CP062811:c4129270-4128956 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T176653 NZ_CP082862:1669853-1670716 [Cupriavidus pauculus]
ATGAGTGCGGCCACCCCAAATGTCGCGGCATCCGTCCGTGCGCGTCTGCTCAATGTCGCCAAGGCGCAAGGCGTCGATTT
CAATCAGGTGCTGGTGCGCTTCGCCTTGGAGCGCATCCTCTATCGGCTGACCCAGTCGCCGCACGCGGACCGTTTTCTGC
TCAAAGGTGCGCTGCTGTTCACGCTCTGGTACGACATGCCACACCGGGCCACACGCGACGCCGACCTGTTGGGTTTCGGT
GCTAGTGATCTGGAGTCAGTGGCCCAAGTATTCCGGGACATCGCAGCTGTACCGGTCGACGACGGCATCGTGTTCGACCC
AGCCTCGGTCACAGTCGAGGAGATCCGCAAGGAAGCTGGCTACGGCGGCGTTCGCGTGATGATTGCGGGCGATCTGGCCA
GAGCACGTTGCAAGACCCAAATCGATGTCGGTTTCGGCGACGCCGTCACCCCGGCGCCTGTTGATTCGGTCTACCCAGTA
CTGCTGGAGGAAATGCCCGCGCCACGGCTGCGGGCCTACCCAACGTACACCGTCATCGCGGAAAAGCTCCACGCCATCGC
ATTGCTGGGCATGACCAACAGCCGCCTGAAGGACTACTTCGATCTGTCGGTGCTGTTGGAACGAGAAACTCTGGACACTG
ATCTGCTGGCCCAAGCGATCAAGGCCACATTCGAAAGACGCGGCATGGCGGTTCCTGACGCGCTGCCAGTCGGCCTGACG
GACGAGTTTGCGCACGACGCTTCGCGCCAGGCGCTGTGGCTGGCATTTCTCAAGAAGAACGAACTTCCGCCAGAACCCTT
GCCCGCCATCGTTGGCCGCTTGAGCGTGGCATTGGCCCCCGCGCTGAACGTCGTCGCTCGTTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8599.64 Da
• Isoelectric Point: 6.9791

>AT176653 WP_000125565.1 NZ_CP062811:c4129506-4129273 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRRF

Nucleotide

• Length: 234 bp

>AT176653 NZ_CP082862:1669251-1669856 [Cupriavidus pauculus]
ATGCTCTCCAGTACCCACAATCAACGCGTCCTCGATCTGGCCAGCCAGAAGGGGCTACTGCGCGCCAGCGATCTGGACGC
CATCAGTGCGCCTCGGGTCGTTCTGACGCGCCTGACCGCTGCGGGTCTACTAGAGAAGGTGGGGCGTGGCCTGTACCGTC
TGCCGGATGCACAGGTTTCGGAATTCGAGAGCCTTGGCACCATCGCCACCAAGGTACCTCAAGCGGTGTTCTGCCTGCTT
ACAGCGCTGCAGTTTCATGAGCTGACCACACAGCTGCCGCGTCAGATCTGGATCGCCATGCCTCGGGGCAGCCACCCGCC
CCGGGTCGACTACCCGCCCGTCAAGATGGTGCAGTTCACGGGTGATGCCTACTCGGCAGGGATTGAGGAAGTCGAACGCG
ACGGTGTCAGGCTCCGGGTGTACGGCGTGGCCAAGACCGTGGCGGACTGCTTCAAGCACCGCAACAAGATCGGGCTGGAT
GTGGCGCTGGAGGCGCTGAAGGACGCTCGGGCGCGCAGCAAGGCCTCCGTCGACGACATCTGGCGTTTCGCCAAGATATG
CCGCGTGGCCAATGTGATGCGCCCCTACCTGGAGAGTGTCGGATGA

Structures

Antitoxin

• AlphaFold DB: A0A090NGC7