176650

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 3882586..3883265 Replicon chromosome
Accession NZ_CP062811
Organism Escherichia coli strain Res13-Sevr-PER07-33

Toxin (Protein)


Gene name ykfI Uniprot ID S1FHS4
Locus tag IMQ59_RS18540 Protein ID WP_000854680.1
Coordinates 3882924..3883265 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID S1EWR7
Locus tag IMQ59_RS18535 Protein ID WP_000070396.1
Coordinates 3882586..3882903 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMQ59_RS18490 3878024..3878845 + 822 WP_021574424.1 DUF945 domain-containing protein -
IMQ59_RS18495 3879062..3879763 + 702 WP_021574425.1 WYL domain-containing protein -
IMQ59_RS18500 3879804..3880040 + 237 WP_001144031.1 hypothetical protein -
IMQ59_RS18505 3880040..3880477 + 438 WP_016154551.1 hypothetical protein -
IMQ59_RS18510 3880506..3880973 + 468 WP_001547765.1 hypothetical protein -
IMQ59_RS18515 3881050..3881289 + 240 WP_000194654.1 DUF905 domain-containing protein -
IMQ59_RS18520 3881387..3881845 + 459 WP_000211838.1 antirestriction protein -
IMQ59_RS18525 3881861..3882337 + 477 WP_000811693.1 RadC family protein -
IMQ59_RS18530 3882346..3882567 + 222 WP_000691994.1 DUF987 domain-containing protein -
IMQ59_RS18535 3882586..3882903 + 318 WP_000070396.1 type IV toxin-antitoxin system antitoxin YafW Antitoxin
IMQ59_RS18540 3882924..3883265 + 342 WP_000854680.1 type IV toxin-antitoxin system toxin YkfI Toxin
IMQ59_RS18550 3883837..3885090 - 1254 WP_000893266.1 glutamate-5-semialdehyde dehydrogenase -
IMQ59_RS18555 3885102..3886205 - 1104 WP_001285288.1 glutamate 5-kinase -
IMQ59_RS18560 3886493..3887548 + 1056 WP_000749881.1 phosphoporin PhoE -
IMQ59_RS18565 3887587..3887988 - 402 WP_000174677.1 sigma factor-binding protein Crl -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12892.95 Da        Isoelectric Point: 9.6543

>T176650 WP_000854680.1 NZ_CP062811:3882924-3883265 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T176650 NZ_CP082860:1922978-1923081 [Enterobacter ludwigii]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11982.62 Da        Isoelectric Point: 7.0270

>AT176650 WP_000070396.1 NZ_CP062811:3882586-3882903 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT176650 NZ_CP082860:c1923095-1922943 [Enterobacter ludwigii]
GCTTTCAATCAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCG
CTAAAAGTTGGCATTAATGCAGGCTAAGTCGCCTTGCCTTTTAAGAATAGATGACGACGCCAGGTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHS4

Antitoxin

Source ID Structure
AlphaFold DB S1EWR7

References