Detailed information of TA system TA176647

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2530071..2530709
• Replicon: chromosome
• Accession: NZ_CP062811
• Organism: Escherichia coli strain Res13-Sevr-PER07-33

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A080J2T0
• Locus tag: IMQ59_RS12150
• Protein ID: WP_000813793.1
• Coordinates: 2530533..2530709 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: IMQ59_RS12145
• Protein ID: WP_001270286.1
• Coordinates: 2530071..2530487 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMQ59_RS12125	2525223..2526164	-	942	WP_001773580.1	ABC transporter permease	-
IMQ59_RS12130	2526165..2527178	-	1014	WP_000220411.1	ABC transporter ATP-binding protein	-
IMQ59_RS12135	2527196..2528341	-	1146	WP_001773579.1	ABC transporter substrate-binding protein	-
IMQ59_RS12140	2528586..2529992	-	1407	WP_000760655.1	PLP-dependent aminotransferase family protein	-
IMQ59_RS12145	2530071..2530487	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
IMQ59_RS12150	2530533..2530709	-	177	WP_000813793.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
IMQ59_RS12155	2530931..2531161	+	231	WP_000494244.1	YncJ family protein	-
IMQ59_RS12160	2531253..2533214	-	1962	WP_001773577.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
IMQ59_RS12165	2533287..2533823	-	537	WP_000429143.1	DNA-binding transcriptional regulator SutR	-
IMQ59_RS12170	2533915..2535090	+	1176	Protein_2384	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	sodB / fimA / espR1 / rhs/PAAR / vgrG/tssI	2260492..2580198	319706
	-	flank	IS/Tn	-	-	2535130..2536278	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6724.83 Da
• Isoelectric Point: 10.9223

>T176647 WP_000813793.1 NZ_CP062811:c2530709-2530533 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGMRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T176647 NZ_CP082857:1835515-1835913 [Haemophilus aegyptius]
GTGTTGAAATATATGCTTGATACTAACATTGTGATTTACGTGATTAAGCGTAGGCCATTGGAAATTCTTTCTCGCTTTAA
TCAAAATGCAGGAAAAATGTGCGTGAGTAGCATTACTGTGGCTGAACTCTATTATGGAGCTGAAAAAAGCGAATATCCCG
AAAGAAACATTGCGGTTATTGAAGATTTCTTATCACGCCTCACCATTTTAGATTACCAACCAAAGCATGCAGCTCATTTC
GGCAATATTAAAGCTGAACTCTCTAAACAAGGGAAATTGATTGGCGAAAATGATATTCATATAGCTGCTCATGCTCGTAG
TGAAGGGTTAATTCTGGTTAGTAACAATTTAAGAGAATTTGAGCGAGTTATAGCATTACGTACAGAAAATTGGGTGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT176647 WP_001270286.1 NZ_CP062811:c2530487-2530071 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT176647 NZ_CP082857:1835282-1835515 [Haemophilus aegyptius]
ATGATTGAAGCCTCTGTTTTTATGACTAATCGAAGTCAAGCTGTGCGTTTGCCCGCTGAAGTTCGCTTTTCTGAAGAAAT
CAAAAAATTATCGGTTCGTGTATCGGGCAGTGATCGGATTTTATCGCCTCTCAACCAATCTTGGGATAGTTTTTTCTTGA
ATGATCAAGCAGTTAGCGATGATTTTATGAATGAACGAGAAATTGCATTTCAACCAGAGCGTGAGGCTTTATAG

Structures

Toxin

• AlphaFold DB: A0A080J2T0