Detailed information of TA system TA176621

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4879042..4880723
• Replicon: chromosome
• Accession: NZ_CP062792
• Organism: Klebsiella pneumoniae strain 33Kpn12

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: J2XCI1
• Locus tag: IFY65_RS24155
• Protein ID: WP_004146663.1
• Coordinates: 4879042..4879959 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: IFY65_RS24160
• Protein ID: WP_004177809.1
• Coordinates: 4879965..4880723 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IFY65_RS24135	4874348..4875868	+	1521	WP_192906193.1	sugar ABC transporter ATP-binding protein	-
IFY65_RS24140	4875865..4876863	+	999	WP_032420804.1	ribose ABC transporter permease	-
IFY65_RS24145	4876894..4877835	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
IFY65_RS24150	4877910..4878917	+	1008	WP_004178300.1	carbohydrate kinase family protein	-
IFY65_RS24155	4879042..4879959	-	918	WP_004146663.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
IFY65_RS24160	4879965..4880723	-	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
IFY65_RS24165	4880809..4881537	-	729	WP_023288135.1	response regulator transcription factor	-
IFY65_RS24170	4881810..4883957	+	2148	WP_012737173.1	formate dehydrogenase H subunit alpha, selenocysteine-containing	-
IFY65_RS24175	4884187..4884873	+	687	WP_004151733.1	sel1 repeat family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34659.10 Da
• Isoelectric Point: 5.4707

>T176621 WP_004146663.1 NZ_CP062792:c4879959-4879042 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIAYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T176621 NZ_CP082846:c4195728-4195297 [Mycolicibacterium smegmatis]
ATGTTCCTGCTAGACGTGAACGTCGTACTTGCGGCATACCGCGCAGACCACCCAGATCACGCCACCGTGCGTCCGTGGTT
CGATCGCCTGCTCGATGGCGACGGACCGTTCACCGTCCCGGTACAGGTGTGGGCGTCGTTCCTGCGATTGGTAACCAATC
GGCGTATCTTCCCAGTCCCCACACCCCGTGACGATGCGTTCGCGTTCATCGAAGCCGTCAACGGCCAACCGCACCACGTG
CCGGTAAGCGCGGGCCCGCGGCACCTTGCGCTGCTGCACCAACTTTGCGACGAAGGCGATGCCTCCGGCGACCTGATCCC
CGATGCAGTGCTCGCGGCCATCGCCCACGAACATCATTGCGACGTGGCGACTCTCGATCGGGACTTCGCCCGATTCACCA
CGGTCCGTCATCTGAGGCCGAGTCTGGACTGA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT176621 WP_004177809.1 NZ_CP062792:c4880723-4879965 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT176621 NZ_CP082846:c4195994-4195737 [Mycolicibacterium smegmatis]
GTGCGCACCACGATCGCGATCGCCGATGAATTACTGGCCGCGGCCAAGCGCCGAGCCCGCCAGCGTGGCCAGTCATTGGG
CTCGGTGATCGAGGATGCTCTGCGCCGCGAACTGGCTGCCGATCAGGAAGCGGCGCCACGTCCAGAGGTTCCGGTATTCA
CCGACGGCACCGGGCCGCGTGCCGGTATCGACCTACGGTCGGCCCGCGCACTTCACGAAGTCCTCGACGAAGGCCTCGAC
CTCAACGCGCTCAAGTAG

Structures

Toxin

• AlphaFold DB: J2XCI1

Antitoxin

• AlphaFold DB: A6TH05