Detailed information of TA system TA176604

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 4587908..4588544
• Replicon: chromosome
• Accession: NZ_CP062790
• Organism: Cytobacillus oceanisediminis strain YPW-V2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: W7KPR3
• Locus tag: IIE26_RS22895
• Protein ID: WP_009336311.1
• Coordinates: 4588194..4588544 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A160MHA2
• Locus tag: IIE26_RS22890
• Protein ID: WP_026041666.1
• Coordinates: 4587908..4588189 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IIE26_RS22870	4583804..4584529	-	726	WP_192908030.1	rhomboid family intramembrane serine protease	-
IIE26_RS22875	4584694..4585047	+	354	WP_009336315.1	holo-ACP synthase	-
IIE26_RS22880	4585192..4586205	+	1014	WP_019381248.1	outer membrane lipoprotein carrier protein LolA	-
IIE26_RS22885	4586260..4587531	+	1272	WP_009336313.1	alanine racemase	-
IIE26_RS22890	4587908..4588189	+	282	WP_026041666.1	CopG family ribbon-helix-helix protein	Antitoxin
IIE26_RS22895	4588194..4588544	+	351	WP_009336311.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
IIE26_RS22900	4588926..4589759	+	834	WP_009336310.1	STAS domain-containing protein	-
IIE26_RS22905	4589756..4590118	+	363	WP_009336309.1	STAS domain-containing protein	-
IIE26_RS22910	4590123..4590524	+	402	WP_009336308.1	anti-sigma regulatory factor	-
IIE26_RS22915	4590536..4591546	+	1011	WP_019381250.1	PP2C family protein-serine/threonine phosphatase	-
IIE26_RS22920	4591607..4591939	+	333	WP_009336305.1	anti-sigma factor antagonist	-
IIE26_RS22925	4591936..4592409	+	474	WP_009336304.1	anti-sigma B factor RsbW	-
IIE26_RS22930	4592387..4593181	+	795	WP_009336303.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13006.03 Da
• Isoelectric Point: 4.8998

>T176604 WP_009336311.1 NZ_CP062790:4588194-4588544 [Cytobacillus oceanisediminis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEAVQISLGLIEF

Nucleotide

• Length: 351 bp

>T176604 NZ_CP082840:c4797999-4797466 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10817.30 Da
• Isoelectric Point: 7.2025

>AT176604 WP_026041666.1 NZ_CP062790:4587908-4588189 [Cytobacillus oceanisediminis]
VSDSSTTTEIMVKLPQHLLTELDGFAKQENVNRSEFIYQATKMYLRERKKRQIRESMRRGYMEMAKINLTIASEAFQAEY
EAEHTVERLVSGG

Nucleotide

• Length: 282 bp

>AT176604 NZ_CP082840:c4798276-4798010 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: A0A169FD46

Antitoxin

• AlphaFold DB: A0A160MHA2